Wynendaele, EvelienVerbeke, FrederickD’Hondt, MatthiasHendrix, AnVan de Wiele, ChristopheBurvenich, ChristianPeremans, KathelijneDe Wever, OlivierBracke, MarcDe Spiegeleer, Bart2017-03-032017-03-032015-02Wynendaele, E, Verbeke, F, D’Hondt, M, Hendrix, A, Van de Wiele, C, Burvenich, C, Peremans, K, De Wever, O, Bracke, M & De Spiegeleer, B 2015, 'Crosstalk between the microbiome and cancer cells by quorum sensing peptides', Peptides, vol. 64, pp. 40-48.0196-9781 (print)1873-5169 (online)10.1016/j.peptides.2014.12.009http://hdl.handle.net/2263/59248To date, the precise role of the human microbiome in health and disease states remains largely undefined. Complex and selective crosstalk systems between the microbiome and mammalian cells are also not yet reported. Research up till now mainly focused on bacterial synthesis of virulence factors, reactive oxygen/nitrogen species (ROS/RNS) and hydrogen sulphide, as well as on the activation of exogenous mutagen precursors by intestinal bacteria. We discovered that certain quorum sensing peptides, produced by bacteria, interact with mammalian cells, in casu cancer cells: Phr0662 (Bacillus sp.), EntF-metabolite (Enterococcus faecium) and EDF-derived (Escherichia coli) peptides initiate HCT-8/E11 colon cancer cell invasion, with Phr0662 also promoting angiogenesis. Our findings thus indicate that the human microbiome, through their quorum sensing peptides, may be one of the factors responsible for cancer metastasis.en© 2014 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Peptides. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Peptides, vol. 64, pp. 40-48, 2015. doi : 10.1016/j.peptides.2014.12.009.Quorum sensing peptidesColon cancerMetastasisMicrobiomeAngiogenesisPostprint Article