Nothling, Johan OlivietteDe Cramer, Kurt Guido Mireille2021-02-042021-02-042019-10-22Nöthling, J.O. & De Cramer, K.G.M., 2019, ‘Comparison of progesterone assay by chemiluminescence or radioimmunoassay for clinical decision-making in canine reproduction’, Journal of the South African Veterinary Association 90(0), a1730. https://DOI.org/10.4102/jsava.v90i0.1730.1019-9128 (print)2224-9435 (online)10.4102/jsava.v90i0.1730http://hdl.handle.net/2263/78246The Coat-A-Count® radioimmunoassay has been long and widely used to determine the concentration of progesterone in serum or plasma of bitches (progRIA), but was discontinued in 2014. The Immulite® 1000 LKPG1 chemiluminescence immunoassay has gained prominence since 2003 to determine the concentration of progesterone in serum of bitches, but the assay changed in 2012 (Immulite® 1000 LKPW1). This study assessed the feasibility of using Immulite® 1000 LKPW1 (progImm) to estimate the time of clinically relevant events during oestrus and compared progRIA and progImm 2 and 3 days after the first or only day of the luteinizing hormone surge (LH1). ProgImm first exceeded 5.1 nmol/L on the same day that progRIA first exceeded 6 nmol/L, a proxy for the occurrence of the LH surge, or the day before in 28 of 31 (90%) of oestrous periods. ProgImm first exceeded 13.6 nmol/L on the same day that progRIA first exceeded 16 nmol/L (a proxy for the day of ovulation) or the day before in 34 of 35 (97%) oestrous periods. ProgImm first exceeded 5.4 nmol/L on LH1 or the day before in 24 of 25 (95%) of oestrous periods. The median of progImm 2 days after LH1 was 1.2 nmol/L lower than the 10.7 nmol/L of progRIA (p = 0.001). The mean of progImm 3 days after LH1 was 2.2 nmol/L lower than the 19.0 nmol/L of progRIA (p < 0.001). In conclusion, the days on which progImm first exceeded 5.1 nmol/L, 13.6 nmol/L and 5.4 nmol/L effectively estimate the days on which progRIA reached 6 nmol/L or 16 nmol/L or LH1.en© 2019. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.ProgesteroneBitchRadioimmunoassayCoat-a-countImmuliteLuteinizing hormoneArticle