Du Preez, J.L.Lotter, A.P.Verwoerd, Daniel Wynand2013-08-152013-08-1520131996Du Preez, JL & Lotter, AP 1996, 'Solid-phase extraction and HPLC determination of levamisole hydrochloride in sheep plasma’. Onderstepoort Journal of Veterinary Research, vol. 63, no. 3, pp. 209-211.0330-2465http://hdl.handle.net/2263/22040The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.The anthelmintic, levamisole, was determined in sheep plasma by means of ion-pair solid-phase extraction (SPE) and reverse-phase liquid chromatography. The SPE columns were conditioned with 2 ml of methanol followed by 1 ml of octane sulphonic-acid buffer. After sample application, the columns were washed with 2 ml of the same buffer, followed by elution with 90/10 acetonitrile: buffer. A phenyl reversephase column (Spherisorb S5 Phenyl, 250 x 4,6 mm) was used with a mobile phase of acetonitrile: 0,005 M of octane sulphonic-acid sodium salt and 0,2% triethylamine in water, pH 3,5, 38/62. Extraction recoveries of 89-94% were achieved over the range from 100-3 750 ng/ml. Accuracy and precision were better than 96% and 2,6%, respectively, over said range, with a limit of quantitation of 50 ng/ml.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineHPLC determinationLevamisole hydrochlorideSheep plasmaSolid-phase extractionVeterinary medicine -- South AfricaArticle