Botha, C.J. (Christoffel Jacobus)Naude, T.W.Swan, Gerry E.Verwoerd, Daniel Wynand2013-11-202013-11-2020131992Botha, CJ, Naude, TW, Swan, GE & Guthrie, AJ 1992, 'Evaluation of the efficacy of d-penicillamine and trientine as copper chelators using an in vitro technique involving ovine red blood cells’, Onderstepoort Journal of Veterinary Research, vol. 59, no. 3, pp. 191-1950330-246571021270477006558662I-7222-2013 7006535470J-6375-20130000-0001-7729-9918O-5991-2014http://hdl.handle.net/2263/32520The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.An in vitro technique for haemolysing ovine red blood cells with copper sulphate was standardized to induce c 50% haemolysis with 0,5 mM CuSO₄ after incubation for 14 h at 38°C. This technique was then applied to test the efficacy of trientine and d-penicillamine in preventing haemolysis. Trientine concentrations of 0,5; 1,0 and 1,5 mM were found to be the most effective (P<0,05) in reducing copper-induced haemolysis. One and 1,5 mM concentrations of d-penicillamine were also effective (P< 0,05), but in this experiment a 0,5 mM concentration failed to protect the erythrocytes.en©South Africa. Dept. of Agricultural Technical Services. ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineSheepVeterinary medicine -- South AfricaArticle