Josemans, A.I.Boomker, Jacob Diederik Frederik2012-03-202012-03-2020122001Zweygarth, E & Josemans, AI 2001, 'A chemically defined medium for the growth of Cowdria ruminantium’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 1, pp. 37-40.0030-2465http://hdl.handle.net/2263/18484The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Chemically defined media, termed SFMC-23 and SFMC-36, were devised for the in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants. Both media were based on Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 (DME/F-12) containing various supplements. Medium SFMC-23 and SFMC-36 supported the long-term growth of the Welgevonden stock of C. ruminantium for a total of 55 and 28 passages, respectively, with regular passage intervals of 3 days. Using SFMC-23, split ratios varied from 5-10, depending on which host cell line was used. Other stocks of C. ruminantium (Sankat, Blaauwkrantz, Senegal) were successfully propagated for a test period of ten passages.© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineChemically defined mediumCowdria ruminantiumIn vitro cultureVeterinary medicine -- South AfricaHeartwater -- South AfricaTick-borne diseases in animalsArticle