Van Niekerk, C.Just, M.C.De Waal, D.T.Verwoerd, Daniel Wynand2013-09-122013-09-1220131995Zweygarth, E, Van Niekerk, C, Just, MC & De Waal, DT 1995, 'In vitro cultivation of a Babesia sp. from cattle in South Africa’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 2, pp. 139-142.0330-2465http://hdl.handle.net/2263/31679The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A South African Babesia sp. of cattle which is as yet unclassified, was continuously cultivated in micro-aerophilous stationary-phase culture. The parasites were resuscitated from a blood stabilate stored in liquid nitrogen. A modified HL-1 medium supplemented with either horse or bovine serum was used. Cultures were initiated in a humidified atmosphere containing 2% 0₂ , 5% C0₂ and 93% N₂ at 37°C. Parasites were detected on Giemsa-stained smears after 2 d in culture. On day 4, the cultures were split at a ratio of 1:2 (v/v) and transferred into a humidified atmosphere of 5% C0₂ in air. Starting from day 6, subcultures were made daily at a ratio of 1:4 (v/v). The percentage of parasitized erythrocytes ranged from 2-5%. Addition of purine bases (hypoxanthine, adenine, adenosine or guanosine) was essential for the continuous propagation of the parasites when bovine, but not horse serum, was used for medium supplementation.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineIn vitroCultivationBabesiaCattleBovineSouth AfricaVeterinary medicine -- South AfricaArticle