Coertse, JessicaMarkotter, WandaLe Roux, KevinStewart, DanielSabeta, Claude TauraiNel, Louis Hendrik2017-03-292017-03-292017-01-31Coertse, J, Markotter, W, le Roux, K, Stewart, D, Sabeta, CT & Nel, LH 2017, 'New isolations of the rabies-related Mokola virus from South Africa', BMC Veterinary Research, vol. 13, art. no. 37, pp. 1-8.1746-614810.1186/s12917-017-0948-0http://hdl.handle.net/2263/59559Additional file 1: Table S1. Primers and PCR conditions for amplification of the Nucleoprotein-, Phosphoprotein-, Matrix protein- and Glycoprotein genes of Mokola virus isolates.Additional file 2: Table S2. Details of sequences used for the Bayesian analysis of the rabies virus positive samples.Additional file 3: Table S3. Details of sequences used for the Bayesian analysis of the new Mokola virus isolates.Additional file 4: Figure S1. Bayesian analysis of the coding region of the Nucleoprotein gene (1353 bp) of all Mokola virus isolates (Additional file 3: Table S3) applying the general time reversible substitution model with invariable sites. Laboratory reference numbers are shown for all sequences, followed by the host species, country of origin (KZN SA: KwaZulu-Natal province, South Africa; EC SA: Eastern Cape province South Africa; ZIM: Zimbabwe; CAR: Central African Republic; NIG: Nigeria) and year of isolation.Additional file 5: Figure S2. Bayesian analysis of the coding region of the Phosphoprotein gene (913 bp) applying the general time reversible substitution model with gamma distribution. Laboratory reference numbers are shown for all sequences, followed by the host species, country of origin (KZN SA: KwaZulu-Natal province, South Africa; EC SA: Eastern Cape province South Africa; ZIM: Zimbabwe; CAR: Central African Republic; NIG: Nigeria) and year of isolation.Additional file 6: Figure S3. Bayesian analysis of the coding region of the Matrix protein gene (609 bp) applying the general time reversible substitution model with gamma distribution. Laboratory reference numbers are shown for all sequences, followed by the host species, country of origin (KZN SA: KwaZulu-Natal province, South Africa; EC SA: Eastern Cape province South Africa; ZIM: Zimbabwe; CAR: Central African Republic; NIG: Nigeria) and year of isolation.Additional file 7: Figure S4. Bayesian analysis of the coding region of the Glycoprotein gene (1569 bp) applying the general time reversible substitution model with gamma distribution and invariable sites. Laboratory reference numbers are shown for all sequences, followed by the host species, country of origin (KZN SA: KwaZulu-Natal province, South Africa; EC SA: Eastern Cape province South Africa; ZIM: Zimbabwe; CAR: Central African Republic; NIG: Nigeria) and year of isolation.Additional file 8: Table S4. Nucleotide identity of the Nucleoprotein gene of all Mokola virus isolates.Additional file 9: Table S5. Nucleotide identity of the Phosphoprotein gene of all Mokola virus isolates.Additional file 10: Table S6. Nucleotide identity of the Matrix protein gene of all Mokola virus isolates.Additional file 11: Table S7. Nucleotide identity of the Glycoprotein gene of all Mokola virus isolates.BACKGROUND : Mokola virus (MOKV) is a rabies-related lyssavirus and appears to be exclusive to the African continent. Only 24 cases of MOKV, which includes two human cases, have been reported since its identification in 1968. MOKV has an unknown reservoir host and current commercial vaccines do not confer protection against MOKV. RESULTS : We describe three new isolations of MOKV from domestic cats in South Africa. Two cases were retrospectively identified from 2012 and an additional one in 2014. CONCLUSIONS : These cases emphasize the generally poor surveillance for rabies-related lyssaviruses and our inadequate comprehension of the epidemiology and ecology of Mokola lyssavirus per se.en© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.LyssavirusRabies-relatedSouth Africa (SA)Mokola virus (MOKV)Article