Honiball, John RobertPepper, Michael SeanPrinsloo, Earl2021-06-292021-06-292021Honiball, J.R., Pepper, M.S. & Prinsloo, E. 2021, 'Step-by-step assembly and testing of a low-cost bioprinting solution for research and educational purposes', MethodsX, vol. 8, 2021, art. 101186, pp. 1-9.2215-0161 (online)10.1016/j.mex.2020.101186http://hdl.handle.net/2263/80634Bioprinting is a rapidly expanding technology with the ability to fabricate in vitro three-dimensional (3D) tissues in a layer-by-layer manner to ultimately produce a living tissue which physiologically resembles native in vivo tissue functionality. Unfortunately, large costs associated with commercially available bioprinters severely limit access to the technology. We investigated the potential for modifying a low-cost commercially available RepRap Prusa iteration 3 (i3) 3D printer with an open-source syringe-housed microextrusion print-head unit (universal paste extruder by Richard Horne, RichRap), that allowed for controlled deposition of cell-laden bioinks and Freeform Reversible Embedding of Suspended Hydrogels (FRESH) method-based printing.en© 2020 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.BioprintingSodium alginateFreeform reversible embedding of suspended hydrogels (FRESH)Additive manufacturing (AM)Article