Zhang, XukeSong, ZitengLi, YingWang, HuimeiZhang, ShaojieReid, Anna-MariLall, NamritaZhang, JieWang, ChunyanLee, DonghoOhizumi, YasushiXu, JingGuo, Yuanqiang2021-07-212021-04Zhang, X.K., Song, Z.T., Li, Y. et al. 2021, 'Cytotoxic and atiangiogenetic xanthones inhibiting tumor proliferation and metastasis from Garcinia xipshuanbannaensis', Journal of Natural Products, vol. 85, nol. 5, pp. 1515-1523.0163-3864 (print)1520-6025 (online)10.1021/acs.jnatprod.0c01354http://hdl.handle.net/2263/80934Eight prenylated xanthones including four new analogues were extracted and purified from the leaves of Garcinia xipshuanbannaensis. Multiple techniques including UV, 1D and 2D NMR, and HRESIMS were used to determine the structures of the isolated xanthones. These xanthones were evaluated for their cytotoxicity toward human cancer cells, and compound 4 exhibited activity against HeLa cells. A cytotoxic mechanism examination revealed the active compound induced cell apoptosis by arresting the cell cycle, increasing the levels of ROS, and inhibiting the expression of p-STAT3 in HeLa cells. In in vivo zebrafish experiments, compound 4 was found to block tumor proliferation and migration and have antiangiogenetic activity, and thus seems worthy of further laboratory evaluation.enThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Natural Products, © 2021 American Chemical Society and American Society of Pharmacognosy, after peer review and technical editing by the publisher. To access https://pubs.acs.org/journal/jnprdf.XanthonesGarcinia xipshuanbannaensisCytotoxicAntiangiogeneticMetastasisTumor proliferationPostprint Article