Kyomuhimbo, Hilda DinahChirwa, Evans M.N.Feleni, UsisiphoBrink, Hendrik Gideon2024-12-122024-12-122024Kyomuhimbo H.D., Chirwa E.M., Feleni U., Brink H.G., 2024, Immobilization of laccase enzyme on zinc oxide and silver doped zinc oxide nanoparticle-chitosan-PVPP composite beads , Chemical Engineering Transactions, 110, 373-378. DOI:10.3303/CET24110063.2283-9216 (online)10.3303/CET24110063http://hdl.handle.net/2263/99896Laccase enzyme has gained popularity due to its wide range of substrates, use of only molecular oxygen as a co-factor and release of water as a by-product. In order to improve its reusability in harsh conditions, various supports have been explored for its immobilization. In this study, laccase was immobilized on zinc oxidechitosan/polyvinylpolypyrolidone (ZnONPs/CS/PVPP) and silver doped zinc oxidechitosan/polyvinylpolypyrolidone (Ag@ZnONPs/CS/PVPP) beads. The Ag@ZnONPs/CS/PVPP beads showed a higher immobilization yield and retained enzyme activity (76.0% and 50.0% respectively) compared to the ZnONPs/CS/PVPP beads (50.4% and 41.7% respectively). The biocatalysts demonstrated improved enzyme stability at higher temperatures and longer storage stability compared to the free enzyme hence show potential for further biotechnological applications.en© 2024, AIDIC Servizi S.r.l. Open Access.Laccase enzymeImmobilizationZinc oxide-chitosan (ZnONPs/CS)Silver-doped beads (Ag@ZnONPs/CS/PVPP)BiocatalystsSDG-09: Industry, innovation and infrastructureSDG-12: Responsible consumption and productionArticle