Adesioye, Fiyinfoluwa AdenikeMakhalanyane, Thulani PeterVikram, SurendraSewell, Bryan T.Schubert, Wolf-DieterCowan, Don A.2018-04-202018-04Adesioye FA, Makhalanyane TP, Vikram S, Sewell BT, Schubert W-D, Cowan DA. 2018. Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family. Appl Environ Microbiol 84:e02695-17. https://doi.org/10.1128/AEM.02695-17.0099-2240 (print)1098-5336 (online)10.1128/AEM.02695-17http://hdl.handle.net/2263/64669Please read abstract in the article.en© 2018, American Society for Microbiology. All Rights Reserved.X-ray crystallographyStructural characterizationSingle-point mutationSalt concentrationMetagenomicsDirected evolutionCatalytic efficienciesBiosynthesisCarbohydratesCatalyst activityCloningCrystal structureEfficiencyEnzymesEstersGenesSodium compoundsThermodynamic stabilityAcetyl xylan esterase (AcXE)Carbohydrate esterase 7 (CE7)Sequence-based metagenomicsThermal stabilityPostprint Article