A direct neurokinin B projection from the arcuate nucleus regulates magnocellular vasopressin cells of the supraoptic nucleus

dc.contributor.authorPineda, Rafael
dc.contributor.authorSabatier, Nancy
dc.contributor.authorLudwig, Mike
dc.contributor.authorMillar, Robert P.
dc.contributor.authorLeng, Gareth
dc.date.accessioned2016-06-22T06:15:24Z
dc.date.issued2016-04
dc.description.abstractCentral administration of neurokinin B (NKB) agonists stimulates immediate early gene expression in the hypothalamus and increases secretion of vasopressin from the posterior pituitary through a mechanism that depends on the activation of neurokinin receptor 3 receptors (NK3R). Here we report that, in the rat, immunoreactivity for NK3R is expressed in magnocellular vasopressin and oxytocin neurones in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of the hypothalamus, and that NKB immunoreactivity is expressed in fibres in close juxtaposition with vasopressin neurones at both of these sites. Retrograde tracing in the rat showed that some NKB-expressing neurones in the arcuate nucleus project to the SON, and in mice, using an anterograde tracing approach, we found that kisspeptin-expressing neurones of the arcuate nucleus, which are known to co-express NKB, project to the SON and PVN. Finally, we show that i.c.v. injection of the NK3R agonist senktide potently increases the electrical activity of vasopressin neurones in the SON in vivo with no significant effect detected on oxytocin neurons. The results suggest that NKBcontaining neurones in the arcuate nucleus regulate the secretion of vasopressin from magnocellular neurones in rodents, and we discuss the possible significance of this.en_ZA
dc.description.departmentZoology and Entomologyen_ZA
dc.description.embargo2017-04-30
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipThis work was supported by the Newton International Fellowship program awarded to RPR (Ref. NF130516), co-funded by the Royal Society and the British Academy, and the British Society for Neuroendocrinology (Project Support Grant), and supported in part by funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no. 245009 (Neurofast).en_ZA
dc.description.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2826en_ZA
dc.identifier.citationPineda, R, Sabatier, N, Ludwig, M, Millar, RP & Leng, G 2016, 'A direct neurokinin B projection from the arcuate nucleus regulates magnocellular vasopressin cells of the supraoptic nucleus', Journal of Neuroendocrinology, vol. 28, no. 4, pp. 1-10.en_ZA
dc.identifier.issn0953-8194 (print)
dc.identifier.issn1365-2826 (online)
dc.identifier.other10.1111/jne.12342
dc.identifier.urihttp://hdl.handle.net/2263/53293
dc.language.isoenen_ZA
dc.publisherWileyen_ZA
dc.rights© 2015 British Society for Neuroendocrinology. This is the pre-peer reviewed version of the following article : A direct neurokinin B projection from the arcuate nucleus regulates magnocellular vasopressin cells of the supraoptic nucleus, Journal of Neuroendocrinology, vol.28, no. 4, pp. 1-10, 2016. doi : 10.1111/jne.12342. The definite version is available at : http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2826.en_ZA
dc.subjectElectrophysiologyen_ZA
dc.subjectNeuroanatomyen_ZA
dc.subjectMagnocellular neuronesen_ZA
dc.subjectHypothalamusen_ZA
dc.subjectOxytocinen_ZA
dc.subjectNeurokinin B (NKB)en_ZA
dc.subjectNeurokinin receptor 3 receptors (NK3R)en_ZA
dc.subjectSupraoptic nucleus (SON)en_ZA
dc.subjectParaventricular nucleus (PVN)en_ZA
dc.titleA direct neurokinin B projection from the arcuate nucleus regulates magnocellular vasopressin cells of the supraoptic nucleusen_ZA
dc.typePostprint Articleen_ZA

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