Extraction and film properties of kafirin from coarse sorghum and sorghum DDGS by percolation

dc.contributor.authorMuhiwa, Peter J.
dc.contributor.authorTaylor, Janet
dc.contributor.authorTaylor, J.R.N. (John Reginald Nuttall)
dc.contributor.emailjohn.taylor@up.ac.zaen_ZA
dc.date.accessioned2017-08-16T06:59:38Z
dc.date.available2017-08-16T06:59:38Z
dc.date.issued2017-07
dc.description.abstractThe high cost of kafirin and zein restricts their use for bioplastic and food applications. Effective, simple, and rapid kafirin/zein isolation processes are required. Here a percolation-type aqueous ethanol solvent extraction process from coarse meals (grits) and coarse sorghum distillers dried grains and solubles (DDGS) for kafirin and zein isolation employing a low ratio of extractant to meal (2.5:1) was investigated, which is potentially applicable in the grain bioethanol industry. Postextraction filtration times were more than twice as fast using coarse meals compared with fine flours. Washing the meals prior to extraction to remove starch improved protein preparation purity to 73–85% compared with 68–72% for unwashed meals. Hence, no subsequent filtration or centrifugation step is required to clean up the kafirin/zein solution prior to solvent evaporation. With a single extraction step, kafirin/zein yields were 48% (protein basis) for DDGS and 53–70% for washed sorghum/maize meals. Cast films were used as a model bioplastic system to evaluate extracted kafirin/zein functional properties. DDGS kafirin films had rough surfaces but had the lowest water uptake and in vitro digestibility, owing to heat-induced disulfide crosslinking during DDGS processing. Extraction by percolation using coarse meal/DDGS has potential to improve kafirin/zein viability.en_ZA
dc.description.departmentFood Scienceen_ZA
dc.description.librarianhj2017en_ZA
dc.description.sponsorshipSA Technology Innovation Agency seed fund granten_ZA
dc.description.urihttp://cerealchemistry.aaccnet.orgen_ZA
dc.identifier.citationMuhiwa, P.J., Taylor, J. & Taylor, J.R.N. 2017, 'Extraction and film properties of kafirin from coarse sorghum and sorghum DDGS by percolation', Cereal Chemistry, vol. 94, no. 4, pp. 693-698.en_ZA
dc.identifier.issn0009-0352
dc.identifier.other10.1094/CCHEM-01-17-0003-R
dc.identifier.urihttp://hdl.handle.net/2263/61689
dc.language.isoenen_ZA
dc.publisherAmerican Association of Cereal Chemistsen_ZA
dc.rights© 2017 AACC International, Inc.en_ZA
dc.subjectDistillers dried grains and solubles (DDGS)en_ZA
dc.subjectBehavioren_ZA
dc.subjectProductsen_ZA
dc.subjectGlutenen_ZA
dc.subjectDigestibilityen_ZA
dc.subjectFunctionalityen_ZA
dc.subjectZeinen_ZA
dc.subjectProteinen_ZA
dc.subjectGamma kafirinen_ZA
dc.subjectSolvent extractionen_ZA
dc.subjectDry-milled cornen_ZA
dc.subjectPercolationen_ZA
dc.subjectSorghumen_ZA
dc.subjectCoarse sorghumen_ZA
dc.subjectKafirinen_ZA
dc.subjectFilm propertiesen_ZA
dc.subjectExtractionen_ZA
dc.titleExtraction and film properties of kafirin from coarse sorghum and sorghum DDGS by percolationen_ZA
dc.typePreprint Articleen_ZA

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