Molecular sexing of African rhinoceros

dc.contributor.authorPeppin, Lindsay
dc.contributor.authorMcEwing, Ross
dc.contributor.authorOgden, Rob
dc.contributor.authorHermes, Robert
dc.contributor.authorHarper, Cindy Kim
dc.contributor.authorGuthrie, Alan John
dc.contributor.authorCarvalho, Gary R.
dc.date.accessioned2010-03-31T08:12:56Z
dc.date.available2010-03-31T08:12:56Z
dc.date.issued2009-04
dc.description.abstractWe report the development of a fast and reliable PCR-based method for sex identification of African Rhinoceros (Ceratotherium simum and Diceros bicornis) that could easily be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. In both species, the amplified ZFX and ZFY amplicons differ in size by 7 bp and can thus be differentiated by capillary electrophoresis. Blood, tissue, horn, and faecal samples were correctly sexed using this method. Cross species testing also demonstrated that this method could be used to sex Indian rhinoceros (Rhinoceros unicornis) samples.en
dc.description.sponsorshipObjective 1—European Social Fund.en
dc.identifier.citationPeppin, L, McEwing, R, Ogden, R, Hermes, R, Harper, C, Guthrie, AJ & Carvalho, GR 2009, 'Molecular sexing of African rhinoceros', Conservation Genetics, doi:10.1007/s10592-009-9912-2. [http://www.springerlink.com/content/105709/]en_US
dc.identifier.issn1566-0621
dc.identifier.other10.1007/s10592-009-9912-2
dc.identifier.other7006535470
dc.identifier.otherJ-6375-2013
dc.identifier.other0000-0001-7729-9918
dc.identifier.urihttp://hdl.handle.net/2263/13758
dc.language.isoenen
dc.publisherSpringeren
dc.rightsSpringer. The original publication is available at www.springerlink.comen
dc.subjectMolecular sex identificationen
dc.subjectCeratotherium simumen
dc.subjectDiceros bicornisen
dc.subjectZinc finger proteinen
dc.subject.lcshPolymerase chain reactionen
dc.subject.lcshRhinoceroses -- Sexingen
dc.subject.lcshWhite rhinoceros -- Sexingen
dc.subject.lcshBlack rhinoceros -- Sexingen
dc.titleMolecular sexing of African rhinocerosen
dc.typePostprint Articleen

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