Investigation of the possible biological activities of a poisonous South African plant ; Hyaenanche globosa (Euphorbiaceae)

dc.contributor.authorMomtaz, Saeideh
dc.contributor.authorLall, Namrita
dc.contributor.authorHussein, Ahmed A.
dc.contributor.authorOstad, Seyed Nasser
dc.contributor.authorAbdollahi, Mohammad
dc.date.accessioned2011-08-04T06:20:01Z
dc.date.available2011-08-04T06:20:01Z
dc.date.issued2010-02
dc.description.abstractThe present study was undertaken to explore the possible biochemical activities of Hyaenanche globosa Lamb. and its compounds. Two different extracts (ethanol and dichloromethane) of four different parts (leaves, root, stem, and fruits) of H. globosa were evaluated for their possible antibacterial, antityrosinase, and anticancer (cytotoxicity) properties. Two pure compounds were isolated using column chromatographic techniques. Active extracts and pure compounds were investigated for their antioxidant effect on cultured ‘Hela cells’. Antioxidant/oxidative properties of the ethanolic extract of the fruits of H. globosa and purifi ed compounds were investigated using reactive oxygen species (ROS), ferric-reducing antioxidant power (FRAP), and lipid peroxidation thiobarbituric acid reactive substance (TBARS) assays. The ethanolic extract of the leaves and fruits of H. globosa showed the best activity, exhibiting a minimum inhibitory concentration (MIC) of 3.1 mg/ ml and a minimum bactericidal concentration (MBC) of 1.56 and 6.2 mg/ml, respectively, against M. smegmatis. The ethanolic extract of the fruits of H. globosa (F.E) showed the highest percentage of inhibitory activity of monophenolase (90.4% at 200 g/ml). In addition, F.E exhibited 50% inhibitory concentration (IC50) of 37.7 g/ml on the viability of ‘HeLa cells’ using cytotoxicity MTT assay. Subsequently, F.E was fractionated using phase-partitioning with n-hexane, ethyl acetate, and n-butanol. The cytotoxicity of these fractions were determined in vitro using different cancer cell lines. The n-hexane fraction exhibited the highest activity of toxicity. Therefore, this fraction was subjected to further separation by chromatographic methods. Two pure compounds known as: ‘Tutin’ and ‘hyenanchin’ were isolated and their structures were determined by NMR spectroscopic methods. Unpredictably, none of them showed signifi cant (P 0.01) inhibition on cell viability/proliferation at the concentrations that were used. F.E showed significant anti-tyrosinase, antibacterial, and cytotoxicity effects, therefore it can be considered as an effective inhibitor alone or in combination with other plant extracts.en
dc.description.urihttp://www.phcog.com/en_US
dc.identifier.citationMomtaz, S, Lall, N, Hussein, A, Ostad, SN & Abdollahi, M 2010, 'Investigation of the possible biological activities of a poisonous South African plant ; Hyaenanche globosa (Euphorbiaceae)', Pharmacognosy Magazine, vol. 6, no. 21, pp. 34-41.en
dc.identifier.issn0973-1296 (print)
dc.identifier.issn0976-4062 (online)
dc.identifier.other10.4103/0973-1296.59964
dc.identifier.urihttp://hdl.handle.net/2263/16995
dc.language.isoenen_US
dc.publisherMedknow Publications and Media Pvt. Ltd.en_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectHyenanche globosaen
dc.subjectHyenanchinen
dc.subjectTutinen
dc.subjectCytotoxicityen
dc.subjectReactive oxygen speciesen
dc.subject.lcshAntibacterial agentsen
dc.subject.lcshAntioxidantsen
dc.subject.lcshChemical inhibitorsen
dc.subject.lcshPoisonous plants -- South Africaen
dc.titleInvestigation of the possible biological activities of a poisonous South African plant ; Hyaenanche globosa (Euphorbiaceae)en
dc.typeArticleen

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