Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks
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Date
Authors
Lukusa, Kambulu
Kabuba, John
Journal Title
Journal ISSN
Volume Title
Publisher
Wolters Kluwer- Medknow
Abstract
OBJECTIVE : To determine the effect of oral selenium supplementation
and semen collection methods on various post thaw semen quality
parameters in Saanen bucks.
METHODS : Sixteen healthy bucks were divided into two equal
groups (n=8 each). The treatment group received selenium at 10-
day intervals for three months. Sperm kinematic parameters,
morphological parameters, mitochondrial membrane potential,
plasma membrane functionality, and sperm DNA integrity were
evaluated weekly pre and post-cryopreservation.
RESULTS : The mean percentages of the morphological abnormalities
were significantly lower in the selenium-supplemented samples
when semen was collected by using artificial vagina method
(P<0.05). Proximal droplet defects were significantly lower in the
selenium supplementation group when semen was collected by
electro-ejaculation (P<0.05). Post-thaw sperm parameters such as
total motility and progressive motility were significantly higher
when semen was obtained by artificial vagina in the seleniumsupplemented
bucks compared to the electro-ejaculation and the
control groups (P<0.05). The sperm kinematic parameters such as
curvilinear velocity, average path velocity, and amplitude of lateral
head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks (P<0.05).
The percentages of sperm with intact and functional plasma
membrane and functional mitochondria were significantly higher in
the selenium-supplemented samples collected with artificial vagina
compared to the electro-ejaculation method and the control groups
(P<0.05). In vitro fertilizing potential was significantly higher in
the selenium-supplemented samples collected with artificial vagina
compared to the electro-ejaculation method and the control groups,
respectively (P<0.05).
CONCLUSIONS : Oral supplementation of selenium and artificial vagina
semen collection improve post thaw sperm quality parameters of
Saanen buck.
Description
Keywords
Post thaw, Cryopreservation, Saanen buck, Selenium supplementation, Semen collection method, Sperm kinematic parameters, Sperm motility, Sperm quality, Saanen bucks
Sustainable Development Goals
Citation
Lukusa, K. & Kabuba, J. Oral supplementation of selenium
improves post-thaw sperm quality in Saanen bucks. Asian Pacific Journal of Reproduction 2021; 10(4): 187-194.