Evidence of variability in the structure and recruitment of rhizospheric and endophytic bacterial communities associated with arable sweet sorghum (Sorghum bicolor (L) Moench)

dc.contributor.authorRamond, Jean-Baptiste
dc.contributor.authorTshabuse, Freedom
dc.contributor.authorBopda, Cyprien William
dc.contributor.authorCowan, Don A.
dc.contributor.authorTuffin, Marla I.
dc.date.accessioned2016-12-14T07:03:48Z
dc.date.available2016-12-14T07:03:48Z
dc.date.issued2013-11
dc.description.abstractSorghum is the second most cultivated crop in Africa and is a staple food source of many African communities. Exploiting the associated plant growth promoting bacteria (PGPB) has potential as an agricultural biotechnology strategy to enhance sorghum growth, yield and nutritional properties. Here we use Terminal-Restriction Fragment Length Polymorphism (TRFLP) and Denaturing Gradient Gel Electrophoresis (DGGE) to evaluate the factors that potentially shape rhizospheric and endophytic bacterial communities associated with sorghum farmed in South Africa. Microbial diversity was typically higher in the rhizosphere and rhizoplane compared to the endophytic zones (root, shoot and stem). Geographical location was one of the main drivers in describing microbial community assemblages found in rhizospheric and endophytic sorghum-linked niches. NO3-N, total nitrogen and pH were clearly identified as the main abiotic factors shaping sorghum-associated soil communities. Our results also suggest that specific bacterial taxa with potential N-fixing capacities (Acetobacter sp., Azospirillum sp., Pantoea sp., Bacillus sp. and cyanobacteria) are consistently detected in sorghum-created rhizospheric and endophytic environments, irrespective of environmental factor effects.en_ZA
dc.description.departmentGeneticsen_ZA
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipThe South African National Research Foundation (NRF)en_ZA
dc.description.urihttp://link.springer.com/journal/11104en_ZA
dc.identifier.citationRamond, J.-B., Tshabuse, F., Bopda, C.W., Cowan, D.A. & Tuffin, M.I. Evidence of variability in the structure and recruitment of rhizospheric and endophytic bacterial communities associated with arable sweet sorghum (Sorghum bicolor (L) Moench). Plant Soil (2013) 372: 265-278. doi:10.1007/s11104-013-1737-6.en_ZA
dc.identifier.issn0032-079X (print)
dc.identifier.issn1573-5036 (online)
dc.identifier.other10.1007/s11104-013-1737-6
dc.identifier.urihttp://hdl.handle.net/2263/58394
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Springer Science+Business Media Dordrecht 2013. The original publication is available at : http://link.springer.comjournal/11104.en_ZA
dc.subjectCore communityen_ZA
dc.subjectEndosphereen_ZA
dc.subjectRhizospheric environmenten_ZA
dc.subjectSorghumen_ZA
dc.subjectPlant growth promoting bacteria (PGPB)en_ZA
dc.subjectTerminal-restriction fragment length polymorphism (TRFLP)en_ZA
dc.subjectDenaturing gradient gel electrophoresis (DGGE)en_ZA
dc.titleEvidence of variability in the structure and recruitment of rhizospheric and endophytic bacterial communities associated with arable sweet sorghum (Sorghum bicolor (L) Moench)en_ZA
dc.typePostprint Articleen_ZA

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