LukMF' is the major secreted leukocidin of bovine Staphylococcus aureus and is produced in vivo during bovine mastitis

dc.contributor.authorVrieling, Manouk
dc.contributor.authorBoerhout, Eveline M.
dc.contributor.authorVan Wigcheren, Glenn F.
dc.contributor.authorKoymans, Kirsten J.
dc.contributor.authorMols-Vorstermans, Tanja G.
dc.contributor.authorDe Haas, Carla J. C.
dc.contributor.authorAerts, Piet C.
dc.contributor.authorDaemen, Ineke J. J. M.
dc.contributor.authorVan Kessel, Kok P. M.
dc.contributor.authorKoets, Ad P.
dc.contributor.authorRutten, Victor P.M.G.
dc.contributor.authorNuijten, Piet J.M.
dc.contributor.authorVan Strijp, Jos A. G.
dc.contributor.authorBenedictus, Lindert
dc.date.accessioned2017-01-13T06:25:06Z
dc.date.available2017-01-13T06:25:06Z
dc.date.issued2016-11-25
dc.description.abstractStaphylococcus aureus is a major human and animal pathogen and a common cause of mastitis in cattle. S. aureus secretes several leukocidins that target bovine neutrophils, crucial effector cells in the defence against bacterial pathogens. In this study, we investigated the role of staphylococcal leukocidins in the pathogenesis of bovine S. aureus disease. We show that LukAB, in contrast to the γ-hemolysins, LukED, and LukMF′, was unable to kill bovine neutrophils, and identified CXCR2 as a bovine receptor for HlgAB and LukED. Furthermore, we assessed functional leukocidin secretion by bovine mastitis isolates and observed that, although leukocidin production was strain dependent, LukMF′ was most abundantly secreted and the major toxin killing bovine neutrophils. To determine the role of LukMF′ in bovine mastitis, cattle were challenged with high (S1444) or intermediate (S1449, S1463) LukMF′-producing isolates. Only animals infected with S1444 developed severe clinical symptoms. Importantly, LukM was produced in vivo during the course of infection and levels in milk were associated with the severity of mastitis. Altogether, these findings underline the importance of LukMF′ as a virulence factor and support the development of therapeutic approaches targeting LukMF′ to control S. aureus mastitis in cattle.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianam2016en_ZA
dc.description.sponsorshipThis work was supported by the ALTANT program of the Dutch Government.en_ZA
dc.description.urihttp://www.nature.com/scientificreportsen_ZA
dc.identifier.citationVrieling, M., Boerhout, E.M., Van Wigcheren, G.F., Koymans, K.J., Mols-Vorstermans, T.G., De Haas, C.J.C., Aerts, P.C., Daemen, I.J.J.M., Van Kessel, K.P.M, Koets, A.P., Rutten, V.P.M.G., Nuijten, P.J.M., Van Strijp, J.A.G. & Benedictus, L. LukMF′ is the major secreted leukocidin of bovine Staphylococcus aureus and is produced in vivo during bovine mastitis. Scientific Reports. 6, 37759; DOI: 10.1038/srep37759 (2016).en_ZA
dc.identifier.issn2045-2322
dc.identifier.other10.1038/srep37759
dc.identifier.urihttp://hdl.handle.net/2263/58501
dc.language.isoenen_ZA
dc.publisherNature Publishing Groupen_ZA
dc.rights© The Author(s) 2016. This work is licensed under a Creative Commons Attribution License 4.0.en_ZA
dc.subjectCattleen_ZA
dc.subjectStaphylococcus aureusen_ZA
dc.subjectMastitisen_ZA
dc.subjectInfectionen_ZA
dc.subjectLukMF′en_ZA
dc.titleLukMF' is the major secreted leukocidin of bovine Staphylococcus aureus and is produced in vivo during bovine mastitisen_ZA
dc.typeArticleen_ZA

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