A sensitive nested real-time RT-PCR for the detection of Shuni virus

dc.contributor.authorVan Eeden, Charmaine
dc.contributor.authorZaayman, Dewald
dc.contributor.authorVenter, Marietjie
dc.contributor.emailmarietjie.venter@up.ac.zaen_ZA
dc.date.accessioned2015-07-01T04:52:42Z
dc.date.available2015-07-01T04:52:42Z
dc.date.issued2014-01
dc.description.abstractRecently Shuni virus (SHUV) has been identified in clinical cases of neurological disease in horses in South Africa. Being that it was one of the less recognized orthobunyaviruses, with limited clinical descriptions of disease dating back to the 1960s and 1970s, SHUV-specific assays were never developed. In this study, the development of a nested real-time PCR assay is described for the detection of SHUV by means of melt-curve analysis using fluorescence resonance energy transfer (FRET) probe technology. The assay was validated against previously positive clinical specimens and a dilution series of controls. This assay was applied in the screening of 386 clinical specimens of horses with neurological signs. SHUV could be amplified from cell culture, blood and tissue specimens from horses with acute disease. This is a rapid and sensitive assay that may be implemented in both diagnostic and research laboratories.en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipNational Health Laboratory Service (NHLS) pathology and the Global Disease Detection (GDD) programme of the Centres for Disease Control (CDC).en_ZA
dc.description.urihttp://www.elsevier.com/locate/jvirometen_ZA
dc.identifier.citationVan Eeden, C, Zaayman, D & Venter, M 2014, 'A sensitive nested real-time RT-PCR for the detection of Shuni virus', Journal of Virological Methods, vol. 195, no. 1, pp. 100-105.en_ZA
dc.identifier.issn0166-0934
dc.identifier.other10.1016/j.jviromet.2013.10.008
dc.identifier.urihttp://hdl.handle.net/2263/45825
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2013 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, vol. 195, no. 1, pp. 100-105, 2014. doi : 10.1016/j.jviromet.2013.10.008en_ZA
dc.subjectReal-time RT-PCRen_ZA
dc.subjectFRET probesen_ZA
dc.subjectDiagnostic PCRen_ZA
dc.subjectShuni virus (SHUV)en_ZA
dc.subjectfluorescence resonance energy transfer (FRET)en_ZA
dc.titleA sensitive nested real-time RT-PCR for the detection of Shuni virusen_ZA
dc.typePostprint Articleen_ZA

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