Genetic diversity of Mycobacterium tuberculosis isolated from tuberculosis patients in the Serengeti ecosystem in Tanzania

dc.contributor.authorMbugi, Erasto V.
dc.contributor.authorKatale, Bugwesa Z.
dc.contributor.authorSiame, Keith K.
dc.contributor.authorKeyyu, Julius D.
dc.contributor.authorKendall, Sharon L.
dc.contributor.authorDockrell, Hazel M.
dc.contributor.authorStreicher, Elizabeth M.
dc.contributor.authorMichel, Anita Luise
dc.contributor.authorRweyemamu, Mark M.
dc.contributor.emailanita.michel@up.ac.zaen_ZA
dc.date.accessioned2015-05-25T06:57:09Z
dc.date.available2015-05-25T06:57:09Z
dc.date.issued2015-03
dc.description.abstractThis study was part of a larger cross-sectional survey that was evaluating tuberculosis (TB) infection in humans, livestock and wildlife in the Serengeti ecosystem in Tanzania. The study aimed at evaluating the genetic diversity of Mycobacterium tuberculosis isolates from TB patients attending health facilities in the Serengeti ecosystem. DNA was extracted from 214 sputum cultures obtained from consecutively enrolled newly diagnosed untreated TB patients aged 18 years. Spacer oligonucleotide typing (spoligotyping) and Mycobacterium Interspersed Repetitive Units and Variable Number Tandem Repeat (MIRU-VNTR) were used to genotype M. tuberculosis to establish the circulating lineages. Of the214 M. tuberculosis isolates genotyped, 55 (25.7%) belonged to the Central Asian (CAS) family, 52 (24.3%) were T family (an ill-defined family), 38 (17.8%) belonged to the Latin American Mediterranean (LAM) family, 25 (11.7%) to the East-African Indian (EAI) family, 25 (11.7%) comprised of different unassigned (‘Serengeti’) strain families, while 8 (3.7%) belonged to the Beijing family. A minority group that included Haarlem, X, U and S altogether accounted for 11 (5.2%) of all genotypes. MIRU-VNTR typing produced diverse patterns within and between families indicative of unlinked transmission chains. We conclude that, in the Serengeti ecosystem only a few successful families predominate namely CAS, T, LAM and EAI families. Other types found in lower prevalence are Beijing, Haarlem, X, S and MANU. The Haarlem, EAI_Somalia, LAM3 and S/convergent and X2 subfamilies found in this study were not reported in previous studies in Tanzania.en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipWT087546MA and MUHAS Sida Sarec [000/3177].en_ZA
dc.description.urihttp://intl.elsevierhealth.com/journals/tubeen_ZA
dc.identifier.citationMbugi, EV, Katale, BZ, Siame, KK, Keyyu, JD, Kendall, SL, Dockrell, HM, Streicher, EM, Michel, AL, Rweyemamu, MM, Warren, RM, Matee, MI & Van Helden, PD 2015, 'Genetic diversity of Mycobacterium tuberculosis isolated from tuberculosis patients in the Serengeti ecosystem in Tanzania', Tuberculosis, vol. 95, no. 2, pp.170-178.en_ZA
dc.identifier.issn1472-9792 (print)
dc.identifier.issn1873-281X (online)
dc.identifier.other10.1016/j.tube.2014.11.006
dc.identifier.otherN-8996-2014
dc.identifier.other55978917900
dc.identifier.urihttp://hdl.handle.net/2263/45247
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.relation.requiresAdobe Acrobat Readeren
dc.rights© 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license.(http://creativecommons.org/licenses/by/3.0/).en_ZA
dc.subjectGenotypingen_ZA
dc.subjectHumaneanimal interfaceen_ZA
dc.subjectSerengeti ecosystemen_ZA
dc.subjectTuberculosis (TB)en_ZA
dc.subjectMycobacterium tuberculosis (MTB)en_ZA
dc.subject.otherVeterinary science articles SDG-01en_ZA
dc.subject.otherVeterinary science articles SDG-03en_ZA
dc.subject.otherSDG-01: No poverty
dc.subject.otherSDG-03: Good health and well-being
dc.titleGenetic diversity of Mycobacterium tuberculosis isolated from tuberculosis patients in the Serengeti ecosystem in Tanzaniaen_ZA
dc.typeArticleen_ZA

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