Fate of Salmonella Typhimurium in laboratory-scale drinking water biofilms

dc.contributor.authorSchaefer, L.M.
dc.contributor.authorBrözel, Volker Siegfried
dc.contributor.authorVenter, S.N. (Stephanus Nicolaas)
dc.date.accessioned2014-04-01T08:13:14Z
dc.date.issued2013-12
dc.description.abstractInvestigations were carried out to evaluate and quantify colonization of laboratory-scale drinking water biofilms by a chromosomally green fluorescent protein (gfp)-tagged strain of Salmonella Typhimurium. Gfp encodes the green fluorescent protein and thus allows in situ detection of undisturbed cells and is ideally suited for monitoring Salmonella in biofilms. The fate and persistence of non-typhoidal Salmonella in simulated drinking water biofilms was investigated. The ability of Salmonella to form biofilms in monoculture and the fate and persistence of Salmonella in a mixed aquatic biofilm was examined. In monoculture S. Typhimurium formed loosely structured biofilms. Salmonella colonized established multi-species drinking water biofilms within 24 hours, forming micro-colonies within the biofilm. S. Typhimurium was also released at high levels from the drinking water-associated biofilm into the water passing through the system. This indicated that Salmonella could enter into, survive and grow within, and be released from a drinking water biofilm. The ability of Salmonella to survive and persist in a drinking water biofilm, and be released at high levels into the flow for recolonization elsewhere, indicates the potential for a persistent health risk to consumers once a network becomes contaminated with this bacterium.en_US
dc.description.librarianhb2014en_US
dc.description.sponsorshipThis research was supported by a grant from the Water Research Commission of South Africa (WRC K5/1276) to VSB and SNV, and National Research Foundation (NRF) grant 2046811 to VSB. LMS was supported by a scholarship from the NRF of South Africa.en_US
dc.description.urihttp://www.iwaponline.com/jwh/default.htmen_US
dc.identifier.citationSchaefer, LM, Brozel, VS & Venter, SN 2013, 'Fate of Salmonella Typhimurium in laboratory-scale', Journal of Water and Health, vol. 11, no. 4, pp. 629-635.en_US
dc.identifier.issn1477-8920 (print)
dc.identifier.other10.2166/wh.2013.208
dc.identifier.urihttp://hdl.handle.net/2263/37247
dc.language.isoenen_US
dc.publisherIWA Publishingen_US
dc.rights© IWA Publishing 2013. The definitive peer-reviewed and edited version of this article is published in Journal of Water and Health, vol.11, no. 4, pp. 629-635, 2013. doi : 10.2166/wh.2013.208 and is available at : http://www.iwaponline.com/jwh/default.htmen_US
dc.subjectSalmonella Typhimuriumen_US
dc.subjectLaboratory scaleen_US
dc.subjectDrinking wateren_US
dc.titleFate of Salmonella Typhimurium in laboratory-scale drinking water biofilmsen_US
dc.typePostprint Articleen_US

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