Molecular identification of different Theileria and Babesia species infecting sheep in Sudan

dc.contributor.authorEl Imam, Ahmed H.
dc.contributor.authorHassan, Shawgi M.
dc.contributor.authorGameel, Ahmed A.
dc.contributor.authorEl Hussein, Abdelrahim M.
dc.contributor.authorTaha, Khalid M.
dc.contributor.authorOosthuizen, Marinda C.
dc.date.accessioned2016-09-12T14:07:49Z
dc.date.available2016-09-12T14:07:49Z
dc.date.issued2016
dc.description.abstractThe epidemiological aspects of sheep piroplasmosis in Sudan are poorly studied, and further investigations using sensitive and precise techniques are required. In this study, the Reverse Line Blot (RLB) hybridization assay was used to detect and simultaneously differentiate between Theileria and Babesia species. DNA was extracted from blood collected on filter paper (n=219) from apparently healthy sheep from six different geographical localities in Sudan. Results indicated that Theileria ovis (88.6%), T. separata (20.1%), T. lestoquardi (16.4%) and T. annulata (16.4%) DNA could be detected in the blood samples. Single and mixed Theileria infections were detected in 74 (33.8%) and 124 (56.6%) respectively and T. ovis being the most prevalent species in the country. T. ovis and T. separata were reported for the first time in sheep in Sudan.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianam2016en_ZA
dc.description.sponsorshipUniversity of Elimam Elmahadi, Ministry of High Education and Scientific Research, Sudanen_ZA
dc.description.urihttp://www.bioone.org/loi/copaen_ZA
dc.identifier.citationEl Imam, AH, Hassan, SM, Gameel, AA, El Hussein, AM, Taha, KM & Oosthuizen, MC 2016, 'Molecular identification of different Theileria and Babesia species infecting sheep in Sudan', Annals of Parasitology, pp. 62, no. 1, pp. 47-54.en_ZA
dc.identifier.issn2299-0631 (print)
dc.identifier.issn2300-6706 (online)
dc.identifier.other10.17420/ap6201.31
dc.identifier.urihttp://hdl.handle.net/2263/56722
dc.language.isoenen_ZA
dc.publisherPolish Parasitological Societyen_ZA
dc.rights© 2016 Polish Parasitological Societyen_ZA
dc.subjectSudanen_ZA
dc.subjectTheileria annulataen_ZA
dc.subjectTheileria lestoquardien_ZA
dc.subjectTheileria ovisen_ZA
dc.subjectTheileria separataen_ZA
dc.subjectReverse line blot (RLB)en_ZA
dc.titleMolecular identification of different Theileria and Babesia species infecting sheep in Sudanen_ZA
dc.typeArticleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
ElImam_Molecular_2016.pdf
Size:
177.46 KB
Format:
Adobe Portable Document Format
Description:
Article
Download

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles (Veterinary Tropical Diseases)
Research Articles (University of Pretoria)
Simple item page