In vitro cultivation of Babesia occultans

dc.contributor.editorVerwoerd, Daniel Wynand
dc.contributor.upauthorVan Niekerk, C.J.
dc.contributor.upauthorZweygarth, Erich
dc.date.accessioned2013-08-29T11:04:26Z
dc.date.available2013-08-29T11:04:26Z
dc.date.created2012
dc.date.issued1996
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en
dc.description.abstractBabesia occultans, the causative agent of a benign form of cattle babesiosis in South Africa, was continuously cultivated in microaerophilous stationary-phase culture. A modified medium, 199, supplemented with either 40% (v/v) bovine or 40% (v/v) horse serum, was used. Cultures were initiated in a humidified atmosphere containing 2% 0₂, 5% C0₂, and 93% N₂. The highest percentage of parasitized erythrocytes (PPE) reached 4,5% in horse-serum- and 2,4% in bovine-serum-supplemented medium. Parasite suspensions were cryopreserved and successfully resuscitated.en
dc.description.librarianmn2013
dc.identifier.citationVan Niekerk, CJ & Zweygarth, E 1996, 'In vitro cultivation of Babesia occultans’. Onderstepoort Journal of Veterinary Research, vol. 63, no. 3, pp. 259-261.en
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/22096
dc.language.isoenen
dc.publisherPublished by the Agricultural Research Council, Onderstepoort Veterinary Instituteen
dc.rights© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).en
dc.subjectVeterinary medicineen
dc.subjectIn vitro cultivationen
dc.subjectBabesia occultansen
dc.subjectBovine babesiosisen
dc.subjectSouth Africaen
dc.subject.lcshVeterinary medicine -- South Africa
dc.subject.lcshBabesiosis in cattle -- South Africaen
dc.titleIn vitro cultivation of Babesia occultansen
dc.typeArticleen

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1996 Onderstepoort Journal of Veterinary Research, Volume 63, 1996
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