Genetic analysis among selected vernonia lines through seed oil content, fatty acids and RAPD DNA markers

dc.contributor.authorRamalema, S.P.
dc.contributor.authorShimelis, H.
dc.contributor.authorNcube, I.
dc.contributor.authorKunert, Karl J.
dc.contributor.authorMashela, Phatu W.
dc.date.accessioned2010-05-28T06:18:03Z
dc.date.available2010-05-28T06:18:03Z
dc.date.issued2010-01
dc.description.abstractVernonia (Vernonia galamensis) is a new potential industrial oilseed crop. The seeds of this crop contain unusual naturally epoxidised fatty acids which are used in the production of various industrial products. The objective of this study was to evaluate and select vernonia lines in Limpopo province through seed oil content, fatty acid content and RAPD DNA markers. Significant differences were observed for the content of seed oil (22.4 - 29.05%), vernolic acid (73.09 - 76.83%), linoleic acid (13.02 - 14.05%), oleic acid (3.77 - 5.28%), palmitic acid (2.48 - 2.98%) and stearic acid (2.26 - 2.75%). Among the 13 RAPD DNA primers screened, primer OPA10 amplified DNA samples and resulted in 4 distinct groupings among tested lines. Four promising lines were selected; Vge-16, Vge-20, Vge-27 and Vge-32 using seed oil content, fatty acids and RAPD markers. The lines will be used for strategic breeding of vernonia as an alternative industrial oil crop in Limpopo province of South Africa or other similar environments.en
dc.identifier.citationRamalema, SP, Shimelis, H, Ncube, I, Kunert, KK & Mashela, PW 2010, 'Genetic analysis among selected vernonia lines through seed oil content, fatty acids and RAPD DNA markers', African Journal of Biotechnology, vol. 9, no. 2, pp. 117-122. [http://www.academicjournals.org/AJB]en
dc.identifier.issn1684–5315
dc.identifier.urihttp://hdl.handle.net/2263/14138
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.rightsAcademic Journalsen_US
dc.subjectRAPDen
dc.subjectSeed oilen
dc.subject.lcshVernonia -- South Africa -- Limpopoen
dc.subject.lcshFatty acidsen
dc.subject.lcshOilseed plants -- Research -- South Africa -- Limpopoen
dc.subject.lcshOils and fats -- Microbiologyen
dc.subject.lcshGenetic markersen
dc.titleGenetic analysis among selected vernonia lines through seed oil content, fatty acids and RAPD DNA markersen
dc.typeArticleen

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