Ectopic phytocystatin expression leads to enhanced drought stress tolerance in soybean (Glycine max) and arabidopsis thaliana through effects on strigolactone pathways and can also result in improved seed traits

dc.contributor.authorQuain, Marian D.
dc.contributor.authorMakgopa, Matome Eugene
dc.contributor.authorGarcía, Belén Márquez
dc.contributor.authorComadira, Gloria
dc.contributor.authorFernandez-Garcia, Nieves
dc.contributor.authorOlmos, Enrique
dc.contributor.authorSchnaubelt, Daniel
dc.contributor.authorKunert, Karl J.
dc.contributor.authorFoyer, Christine H.
dc.date.accessioned2014-07-04T10:00:43Z
dc.date.issued2014-09
dc.description.abstractEctopic cystatin expression has long been used in plant pest management but the cysteine protease targets of these inhibitors might also have important functions in the control of plant lifespan and stress tolerance that remain poorly characterised. We therefore characterised the effects of expression of the rice cystatin, oryzacystatin-I (OCI), on the growth, development and stress tolerance of crop (soybean) and model (Arabidopsis thaliana) plants. Ectopic OCI expression in soybean enhanced shoot branching and leaf chlorophyll accumulation at later stages of vegetative development significantly and enhanced seed protein contents and decreased the abundance of mRNAs encoding strigolactone synthesis enzymes. The OCI expressing A. thaliana showed a slow growth phenotype, with increased leaf numbers and enhanced shoot branching at flowering. The OCI-dependent inhibition of cysteine proteases enhanced drought tolerance in soybean and A. thaliana, photosynthetic CO2 assimilation being much less sensitive to drought-induced inhibition in the OCI expressing soybean lines. Ectopic OCI expression or treatment with the cysteine protease inhibitor E64 increased lateral root densities in A. thaliana. E64 treatment also increased lateral root densities in the max2-1 mutants that are defective in strigolactone signalling, but not in the max3-9 mutants that are defective in strigolactone synthesis. Taken together, these data provide evidence that OCI-inhibited cysteine proteases participate in the control of growth and stress tolerance through effects on strigolactones. We conclude that cysteine proteases are an important target for manipulation not only to control plant growth, development and stress tolerance, but also seed quality traits.en_US
dc.description.librarianhb2014en_US
dc.description.sponsorshipFP7-PIRSES-GA-2008-230830 (LEGIM), PITN-GA-2008-215174 (Chloroplast Signals; D.S.) and PIIF-GA-2011-299347en_US
dc.description.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1467-7652en_US
dc.identifier.citationQuain, MD, Makgopa, ME, Márquez-García, B, Comadira, G, Fernandez-Garcia, N, Olmos, E, Schnaubelt, D, Kunert, KJ & Foyer, CH 2014, 'Ectopic phytocystatin expression leads to enhanced drought stress tolerance in soybean (Glycine max) and arabidopsis thaliana through effects on strigolactone pathways and can also result in improved seed traits', Plant Biotechnology Journal, vol. 12, no. 7, pp. 903-913.en_US
dc.identifier.issn1467-7644 (print)
dc.identifier.issn1467-7652 (online)
dc.identifier.other10.1111/pbi.12193
dc.identifier.urihttp://hdl.handle.net/2263/40550
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2014 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectSoybeansen_US
dc.subjectTobaccoen_US
dc.subjectArabidopsisen_US
dc.subjectDrought toleranceen_US
dc.subjectSeed qualityen_US
dc.subjectCysteine proteaseen_US
dc.subjectCystatinen_US
dc.subjectStrigolactoneen_US
dc.titleEctopic phytocystatin expression leads to enhanced drought stress tolerance in soybean (Glycine max) and arabidopsis thaliana through effects on strigolactone pathways and can also result in improved seed traitsen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Quain_Ectopic_2014.pdf
Size:
1.25 MB
Format:
Adobe Portable Document Format
Description:
Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: