Genotype composition of populations of grapefruit-cross-protecting citrus tristeza virus strain GFMS12 in different host plants and aphid-transmitted sub-isolates

dc.contributor.authorScott, Katherine Anne
dc.contributor.authorHlela, Quinsile
dc.contributor.authorZablocki, Olivier
dc.contributor.authorRead, David Alan
dc.contributor.authorVan Vuuren, Stephanus
dc.contributor.authorPietersen, Gerhard
dc.contributor.emailgerhard.pietersen@up.ac.zaen_US
dc.date.accessioned2013-05-22T07:29:02Z
dc.date.available2013-05-22T07:29:02Z
dc.date.issued2013-01
dc.description.abstractCitrus tristeza virus (CTV) causes severe losses in grapefruit production in South Africa and requires mild strain cross-protection to maintain production. Unfortunately cross-protection breakdown of the pre4 immunizing CTV grapefruit mild source GFMS12 is prevalent in grapefruit in South Africa. The CTV genotype composition of the GFMS12 population inoculated onto different hosts was determined by sequencing part of ORF1a and the p23 gene of multiple clones from each plant. Analysis of the GFMS12 population in Mexican lime, Marsh and Star Ruby grapefruit varieties, revealed that at least four genotypes occur in the GFMS12 population, and that genotype compositions differed amongst the populations in different host plants. Single aphid transmitted sub-isolates derived from the GFMS12 mother population on Mexican lime appeared to contain three populations of a mixture of VT-like and recombinant B165/VT-like genotypes; a mixture of recombinant RB/VT- and B165/VT-like genotypes; and a single recombinant B165/VT-like genotype. This study underlines the importance of determining the genotype composition of a potential CTV pre-immunizing source on a range of inoculated host species before utilization.en_US
dc.description.librarianhb2013en_US
dc.description.sponsorshipCitrus Research International (CRI) (South Africa).en_US
dc.description.urihttp://www.springerlink.com/content/0304-8608/en_US
dc.identifier.citationScott, KA, Hlela Q, Zablocki, O, Read, D, Van Vuuren, S & Pietersen, G 2013, 'Genotype composition of populations of grapefruit-cross-protecting citrus tristeza virus strain GFMS12 in different host plants and aphid-transmitted sub-isolates', Archives of Virology, vol. 158, no. 1, pp. 27-37.en_US
dc.identifier.issn0304-8608 (print)
dc.identifier.issn1432-8798 (online)
dc.identifier.other10.1007/s00705-012-1450-4
dc.identifier.urihttp://hdl.handle.net/2263/21532
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rights© Springer-Verlag 2012. The original publication is available at www.springerlink.comen_US
dc.subjectCitrus tristeza virus (CTV)en_US
dc.subjectGrapefruit productionen_US
dc.subjectSouth Africaen_US
dc.titleGenotype composition of populations of grapefruit-cross-protecting citrus tristeza virus strain GFMS12 in different host plants and aphid-transmitted sub-isolatesen_US
dc.typePostprint Articleen_US

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