Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC

dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorBagla, Victor Patrick
dc.contributor.authorSteenkamp, Paul Anton
dc.contributor.authorFouche, Gerda
dc.contributor.authorOlivier, J. (Jana), 1948-
dc.contributor.authorEloff, Jacobus Nicolaas
dc.contributor.authorMyer, Martin Steven
dc.contributor.emaillyndy.mcgaw@up.ac.zaen
dc.date.accessioned2014-05-29T09:06:42Z
dc.date.available2014-05-29T09:06:42Z
dc.date.issued2013-12-13
dc.description.abstractBACKGROUND: Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage with commercialization potential. METHODS: Extracts of the dried ground aerial parts were prepared using organic solvents (diethyl ether, dichloromethane/methanol, ethyl acetate and ethanol) and water. These extracts were subjected to HPLC, TLC and bioautography analysis with the aim of linking a range of peaks visualized in HPLC chromatography profiles to antibacterial and antifungal activity of the same extracts. RESULTS: HPLC revealed a group of compounds extracted by more than one solvent. Compounds identified include inositol, caffeic acid, quercetin, kaempferol, apigenin, hymenoxin and oleanolic acid. The organic extracts displayed similar TLC profiles, and bioautography indicated approximately five antibacterial compounds, but only two antifungal compounds in these extracts. Bioautography indicated that cold water extracted the least antimicrobial compounds. CONCLUSIONS: Several previously unknown compounds were identified in Athrixia phylicoides extracts, and bioautography indicated a number of antibacterial and antifungal compounds. There were notable differences in chemical composition and bioactivity between the organic and aqueous extracts. Further research is necessary to fully characterize the active components of the extracts.en
dc.description.librarianam2014en
dc.description.librarianmn2014
dc.description.sponsorshipThe National Research Foundation (NRF) of South Africaen
dc.description.urihttp://www.biomedcentral.com/1472-6882/13/356en
dc.identifier.citationMcGaw, LJ, Bagla, VP, Steenkamp, PA, Fouche, G, Olivier, J, Eloff, JN & Myer, MS 2013, 'Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC', BMC Complementary and Alternative Medicine, vol. 13, art. 356, pp. 1-11.en
dc.identifier.issn1472-6882
dc.identifier.other10.1186/1472-6882-13-356
dc.identifier.other55952645400
dc.identifier.other7005589445
dc.identifier.urihttp://hdl.handle.net/2263/39921
dc.language.isoenen
dc.publisherBioMed Centralen
dc.rights© 2013 McGaw et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution Licenseen
dc.subjectAntibacterialen
dc.subjectAntifungalen
dc.subjectAsteraceaeen
dc.subjectAthrixia phylicoidesen
dc.subjectBioautographyen
dc.subjectHPLCen
dc.subjectZulu teaen
dc.subject.lcshAlternative medicine -- South Africaen
dc.subject.lcshMateria medica, Vegetableen
dc.subject.lcshMedicinal plantsen
dc.titleAntifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLCen
dc.typeArticleen

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