Evolution of H5 highly pathogenic avian influenza: sequence data indicate stepwise changes in the cleavage site

dc.contributor.authorAbolnik, Celia
dc.contributor.emailcelia.abolnik@up.ac.zaen_ZA
dc.date.accessioned2017-08-30T07:10:48Z
dc.date.issued2017-08
dc.description.abstractThe genetic composition of an H5 subtype hemagglutinin gene quasispecies, obtained from ostrich tissues that had been infected with H5 subtype influenza virus was analysed using a next generation sequencing approach. The first evidence for the reiterative copying of a poly (U) stretch in the connecting peptide region in the haemagglutinin cleavage site (HACS) by the viral RNA-dependent RNA polymerase (RdRp) is provided. Multiple non-consensus species of RNA were detected in the infected host, corresponding to likely intermediate sequences between the putative low pathogenic precursor nucleotide sequence of the H5 influenza strain and the highly pathogenic avian influenza virus gene sequence. In silico analysis of the identified RNA sequences predicted that the intermediary H5 sequence PQREKRGLF plays an important role in subsequent mutational events that relocate the HACS coding region from stable base-paired RNA regions to a single-stranded bulge, thereby priming the connecting peptide coding region for RdRp slippage.en_ZA
dc.description.departmentProduction Animal Studiesen_ZA
dc.description.embargo2018-08-30
dc.description.librarianhj2017en_ZA
dc.description.sponsorshipThe National Research Foundation [Grant #82392]en_ZA
dc.description.urihttp://link.springer.com/journal/705en_ZA
dc.identifier.citationAbolnik, C. Evolution of H5 highly pathogenic avian influenza: sequence data indicate stepwise changes in the cleavage site. Archives of Virology (2017) 162: 2219-2230. https://doi.org/10.1007/s00705-017-3337-x.en_ZA
dc.identifier.issn0304-8608 (print)
dc.identifier.issn1432-8798 (online)
dc.identifier.other10.1007/s00705-017-3337-x
dc.identifier.urihttp://hdl.handle.net/2263/62146
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Springer-Verlag Wien 2017. The original publication is available at : http://link.springer.comjournal/705.en_ZA
dc.subjectA virusen_ZA
dc.subjectRNA replicationen_ZA
dc.subjectScreenen_ZA
dc.subjectOutbreaken_ZA
dc.subjectEmergenceen_ZA
dc.subjectRecombinationen_ZA
dc.subjectPolymeraseen_ZA
dc.subjectAvian influenzaen_ZA
dc.subjectHaemagglutinin cleavage site (HACS)en_ZA
dc.subjectRNA-dependent RNA polymerase (RdRp)en_ZA
dc.titleEvolution of H5 highly pathogenic avian influenza: sequence data indicate stepwise changes in the cleavage siteen_ZA
dc.typePostprint Articleen_ZA

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