Effects of moxifloxacin on human neutraphil and T-lymphocyte functions in vitro

dc.contributor.authorPotjo, Moliehi
dc.contributor.authorCockeran, Riana
dc.contributor.authorTheron, Annette J.
dc.contributor.authorFeldman, Charles
dc.contributor.authorAnderson, Ronald
dc.contributor.emailriana.cockeran@up.ac.zaen_US
dc.date.accessioned2011-06-13T10:40:49Z
dc.date.available2011-06-13T10:40:49Z
dc.date.issued2010
dc.description.abstractMoxifloxacin is useful in the treatment of respiratory infections, including community-acquired pneumonia, and also shows promise in the treatment of tuberculosis, a clinical setting which necessitates extended administration of this agent. Relatively little is known, however, about the effects of this agent on the antimicrobial and proliferative activities of human neutrophils and T-lymphocytes, respectively. In the current study, we have investigated the effects of moxifloxacin at therapeutic concentrations and greater (1–20 μg/mL) on cytosolic Ca2+ fluxes, generation of antimicrobial reactive oxygen species (ROS), and release of the primary granule protease, elastase, following activation of the cells with the chemoattractant, fMLP (1 μM), or the phorbol ester, PMA (25 ng/mL), using radiometric, chemiluminescence, and colourimetric procedures, respectively. The effects of moxifloxacin on mitogen-activated proliferation of T cells and expression of the interleukin-2 (IL-2) receptor (CD25) were measured using radiometric and flow cytometric procedures respectively. With the exception of elastase release, which was significantly increased (P < 0.05) by treatment of the cells with moxifloxacin at 10 and 20 μg/mL, none of the other neutrophil or lymphocyte functions was affected by moxifloxacin. These observations suggest that extended use of this agent is unlikely to compromise the protective functions of neutrophils and T-lymphocytes and may even potentiate neutrophilmediated antimicrobial activity by increasing the release of elastase.en_US
dc.identifier.citationPotjo, M, Cockeran, R, Annette, JT, Feldman, C & Anderson, R 2010, 'Effects of moxifloxacin on human neutraphil and T-lymphocyte functions in vitro', Pharmaceuticals, vol. 3, pp. 3570-3580. [http://www.mdpi.com/journal/pharmaceuticals/]en_US
dc.identifier.issn1424-8247
dc.identifier.urihttp://hdl.handle.net/2263/16814
dc.language.isoenen_US
dc.publisherMolecular Diversity Preservation Internationalen_US
dc.rights© 2010 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).en_US
dc.subjectCalciumen_US
dc.subjectCD25en_US
dc.subjectElastaseen_US
dc.subjectN-formyl-L-methionyl-L-leucyl-L-phenylalanineen_US
dc.subjectInterleukin-2en_US
dc.subjectPhorbol myristate acetateen_US
dc.subjectMitogen-activated proliferation;en_US
dc.subjectReactive oxygen speciesen_US
dc.subject.lcshMoxifloxacin -- Physiological effecten
dc.subject.lcshLungs -- Diseases -- Treatmenten
dc.titleEffects of moxifloxacin on human neutraphil and T-lymphocyte functions in vitroen_US
dc.typeArticleen_US

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