Anti‑inflammatory and acetylcholinesterase activity of extract, fractions and five compounds isolated from the leaves and twigs of Artemisia annua growing in Cameroon

dc.contributor.authorChougouo, Rosine D.K.
dc.contributor.authorNguekeu, Yves M.M.
dc.contributor.authorDzoyem, Jean Paul
dc.contributor.authorAwouafack, Maurice D.
dc.contributor.authorKouamouo, Jonas
dc.contributor.authorTane, Pierre
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorEloff, Jacobus Nicolaas
dc.date.accessioned2016-09-27T07:59:27Z
dc.date.available2016-09-27T07:59:27Z
dc.date.issued2016-09-09
dc.description.abstractBACKGROUND : Natural products, including those derived from higher plants have, over the years, contributed greatly to the development of modern therapeutic drugs. Due to the medicinal importance in traditional practice and the diversified biology and chemistry of the constituents from Artemisia spp., the genus has been receiving growing attention. The aim of this study was to investigate the ability of the ethanol extract, four fractions (F1–F4) and five compounds namely artemisinin (1), scopoletin (2), chrysosplenetin (3), eupatin (4) and 3-O-β-d-glucopyranoside of sitosterol (5) isolated from A. annua to modulate the activity of anticholinesterase (AchE) and the production of nitric oxide (NO) in LPS-activated RAW 264.7 macrophages. RESULTS : At the lowest concentration tested (6.25 μg/mL), the crude extract and fraction F2 had the highest NO inhibitory activity (72.39 and 71.00 % inhibition respectively) without significant toxicity on the viability of macrophage cells (93.86 and 79.87 % of cell viability respectively). The crude extract inhibited AchE activity by 71.83 % (at 1 mg/mL) with an IC50 value of 87.43 μg/mL while F2 and F4 were the most active fractions (IC50 values of 36.75 and 28.82 μg/mL). Artemisinin (1) and chrysosplenetin (3) had the highest AChE activity with 71.67 and 80.00 % inhibition (at 0.1 mg/mL) and IC50 values of 29.34 and 27.14 μg/mL, respectively. CONCLUSION : Our results validate the traditional use of A. annua and could help to support the usefulness of this plant in the treatment of inflammatory and neurological disorders especially where nitric oxide and a cholinesterase are involved.en_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.librarianam2016en_ZA
dc.description.sponsorshipThe National Research Foundation (NRF) and Medical Research Council (MRC) provided funding to support this study. The Université des Montagnes provided financial support to phytochemical experimental part of this work.en_ZA
dc.description.urihttp://www.springerplus.comen_ZA
dc.identifier.citationChougouo, RDK, Nguekeu, YMM, Dzoyem, JP, Awouafack, MD, Kouamouo, J, Tane, P, McGaw, JJ & Eloff, JN 2016, 'Anti‑inflammatory and acetylcholinesterase activity of extract, fractions and five compounds isolated from the leaves and twigs of Artemisia annua growing in Cameroon', SpringerPlus, vol. 5, pp. 1-7.en_ZA
dc.identifier.issn2193-1801
dc.identifier.urihttp://hdl.handle.net/2263/57033
dc.language.isoenen_ZA
dc.publisherBioMed Centralen_ZA
dc.rights© 2016 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.en_ZA
dc.subjectArtemisia annuaen_ZA
dc.subjectPhytochemical constituentsen_ZA
dc.subjectNitric oxideen_ZA
dc.subjectAcetylcholinesteraseen_ZA
dc.titleAnti‑inflammatory and acetylcholinesterase activity of extract, fractions and five compounds isolated from the leaves and twigs of Artemisia annua growing in Cameroonen_ZA
dc.typeArticleen_ZA

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