Protection against allergic airway inflammation during the chronic and acute phases of Trichinella spiralis infection

dc.contributor.authorAranzamendi, C.
dc.contributor.authorDe Bruin, A.
dc.contributor.authorKuiper, R.
dc.contributor.authorBoog, C.J.P.
dc.contributor.authorVan Eden, Willem
dc.contributor.authorRutten, Victor P.M.G.
dc.contributor.authorPinelli, E.
dc.date.accessioned2014-10-21T12:37:39Z
dc.date.available2014-10-21T12:37:39Z
dc.date.issued2013-01
dc.description.abstractBACKGROUND : Modulation of the host immune response by helminths has been reported to be essential for parasite survival and also to benefit the host by suppressing inflammatory diseases such as allergies. We have previously shown that excretorysecretory products of Trichinella spiralis muscle larvae have immunomodulatory properties and induce in vitro the expansion of CD4+CD25+FOXP3+ Treg cells in a TGF-b-dependent manner. OBJECTIVE : We aimed at determining the effect of the acute (intestinal) and the chronic (muscle) phase of T. spiralis infection on experimental allergic airway inflammation (EAAI) to Ovalbumin (OVA) and the involvement of Treg cells. METHODS : The chronic phase was established before OVA-sensitization/challenge and the acute phase at two-time points, before and after OVA-sensitization. Mice were infected with 400 T. spiralis larvae and after euthanasia different pathological features of EAAI were measured. Adoptive transfer of CD4+ T cells from Trichinella infected mice to OVA sensitized/challenged recipients was also performed. RESULTS : We found that the chronic as well as the acute phase of Trichinella infection sup-press EAAI as indicated by reduction in airway inflammation, OVA-specific IgE levels in sera, Th2-cytokine production and eosinophils in bronchoalveolar lavage. This protective effect was found to be stronger during the chronic phase and to be associated with increased numbers of splenic CD4 +CD25+FOXP3+ Treg cells with suppressive activity. Adoptive transfer of splenic CD4+ T cells from chronically infected mice with elevated numbers of Treg cells resulted in partial protection against EAAI. CONCLUSIONS AND CLINICAL RELEVANCE : These results demonstrate that the protective effect of T. spiralis on EAAI increases as infection progresses from the acute to the chronic phase. Here, Treg cells may play an essential role in the suppression of EAAI. Elucidating the mechanisms and molecular helminth structures responsible for this regulatory process is relevant to develop alternative tools for preventing or treating allergic asthma.en_US
dc.description.librarianhb2014en_US
dc.description.sponsorshipSOR project S/230166/01 from the Netherlands National Institute for Public Health and the Environment (RIVM)en_US
dc.description.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2222en_US
dc.identifier.citationAranzamendi, C, Bruin, A, Kuiper, R, Boog, CJP, Eden, W, Rutten, VPMG & Pinelli, E 2013,'Protection against allergic airway inflammation during the chronic and acute phases of Trichinella spiralis infection', Clinical & Experimental Allergy, vol. 43, no. 1, pp. 103-115.en_US
dc.identifier.issn0954-7894 (print)
dc.identifier.issn1365-2222 (online)
dc.identifier.other10.1111/cea.12042
dc.identifier.urihttp://hdl.handle.net/2263/42415
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.requiresAdobe Acrobat Readeren
dc.rights© 2012 Blackwell Publishing Ltd. This is the pre-peer reviewed version of the following article : Protection against allergic airway inflammation during the chronic and acute phases of Trichinella spiralis infection,Clinical & Experimental Allergy, vol. 43, no.1, pp. 103-115, 2013. doi :10.1111/cea.12042. The definite version is available at : http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2222.en_US
dc.subjectAllergyen_US
dc.subjectExperimental allergic airway inflammationen_US
dc.subjectHelminthsen_US
dc.subjectImmunomodula-tionen_US
dc.subjectTregsen_US
dc.subjectTrichinella spiralisen_US
dc.titleProtection against allergic airway inflammation during the chronic and acute phases of Trichinella spiralis infectionen_US
dc.typePostprint Articleen_US

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