Effects of growth conditions and incubation times on the expression of antigens of Haemophilus paragallinarum which are detected by monoclonal antibodies

dc.contributor.authorBragg, Robert Richard
dc.contributor.authorCoetzee, L.
dc.contributor.editorVerwoerd, Daniel Wynand
dc.contributor.upauthorVerschoor, Jan Adrianus
dc.date.accessioned2012-12-12T07:02:46Z
dc.date.available2012-12-12T07:02:46Z
dc.date.created2012
dc.date.issued1997
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en_US
dc.description.abstractHaemophilus paragallinarum causes infectious coryza in poultry, and a panel of monoclonal antibodies (Mabs) were established, which detect surface antigens of this bacterium. It was postulated that these Mabs could be used to detect antigenic differences between strains of H. paragallinarum used in infectious coryza (IC) vaccines, and isolates made from the field , from poultry vaccinated against IC. It has previously been reported that in South Africa there are three different Mab patterns that have been common to H. paragallinarum isolates for the past three decades. The effects of different growth conditions such as duration of incubation, inoculum size, levels of NAD or NaCI in the medium, and the pH of the medium on these Mab patterns were investigated. It was found that many different factors appear to influence the expression of the antigens detected by the panel of Mabs. It was found that at different stages during the growth cycles, the isolates could be classified into different Mab groups. It was also found that alteration of the inoculum size resulted in Mab-pattern switches. Addition of extra NaCI to the medium, in order to slow the growth rate, was found to result in Mab-pattern switches. pH was found to have signifcant effects on the levels of expression of the antigens detected by the Mabs, although these changes did not result in Mab-pattern switches. The effects of pH were also found to be highly strain dependent. The use of NAD, rather than sterile chicken serum, in the medium did not significantly alter the levels of expression of these antigens. Alterations of the growth conditions greatly affected the levels of expression of the antigens detected by the Mabs, and were highly strain dependent. It was not possible to predict the effects of a particular growth condition on a particular strain or isolate of H. paragallinarum.en_US
dc.identifier.citationBragg, R.R., Coetzee, L. & Verschoor, J.A. 1997, 'Effects of growth conditions and incubation times on the expression of antigens of Haemophilus paragallinarum which are detected by monoclonal antibodies’. Onderstepoort Journal of Veterinary Research, vol. 64, no. 1, pp. 57-63.en_US
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/20702
dc.language.isoenen_US
dc.publisherPublished by the Agricultural Research Council, Onderstepoort Veterinary Instituteen_US
dc.rights© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).en_US
dc.subjectVeterinary medicineen_US
dc.subjectEffectsen_US
dc.subjectExpression of antigensen_US
dc.subjectGrowth conditionsen_US
dc.subjectHaemophilus paragallinarumen_US
dc.subjectInfectious coryzaen_US
dc.subjectIncubation timesen_US
dc.subjectMonoclonal antibody (MAb)en_US
dc.subject.lcshVeterinary medicine -- South Africa
dc.titleEffects of growth conditions and incubation times on the expression of antigens of Haemophilus paragallinarum which are detected by monoclonal antibodiesen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
9bragg1997.pdf
Size:
425.96 KB
Format:
Adobe Portable Document Format
Description:
Main article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: