Novel two-component system-like elements reveal functional domains associated with restriction-modification systems and paraMORC ATPases in bacteria

dc.contributor.authorBellieny-Rabelo, Daniel
dc.contributor.authorPretorius, Willem J.S.
dc.contributor.authorMoleleki, Lucy Novungayo
dc.contributor.emaillucy.moleleki@up.ac.zaen_ZA
dc.date.accessioned2022-03-09T05:39:59Z
dc.date.available2022-03-09T05:39:59Z
dc.date.issued2021-03
dc.description.abstractTwo-component systems (TCS) are important types of machinery allowing for efficient signal recognition and transmission in bacterial cells. The majority of TCSs utilized by bacteria is composed of a sensor histidine kinase (HK) and a cognate response regulator (RR). In the present study,we report two newly predicted protein domains—both to be included in the next release of the Pfamdatabase: Response_reg_2 (PF19192) andHEF_HK(PF19191)—in bacteriawhich exhibit high structural similarity, respectively, with typical domains of RRs and HKs. Additionally, the genes encoding for the novel predicted domains exhibit a 91.6% linkage observed across 644 genomic regions recovered from 628 different bacterial strains. The remarkable adjacent colocalization between genes carrying Response_reg_2 and HEF_HK in addition to their conserved structural features, which are highly similar to those fromwell-known HKs and RRs, raises the possibility of Response_reg_2 and HEF_HK constituting a new TCS in bacteria. The genomic regions inwhich these predicted two-component systems-like are located additionally exhibit an overrepresented presence of restriction–modification (R–M) systems especially the type II R–M. Among these, there is a conspicuous presence of C-5 cytosinespecific DNAmethylaseswhichmay indicate a functional associationwith the newly discovered domains. The solid presence of R–M systems and the presence of theGHKL family domain HATPase_c_3 across most of the HEF_HK-containing genes are also indicative that these genes are evolutionarily related to the paraMORC family of ATPases.en_ZA
dc.description.departmentBiochemistryen_ZA
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.librarianam2022en_ZA
dc.description.sponsorshipThe National Research Foundation (NRF), the Research Technology Fund and the University of Pretoria Postdoctoral Fellowship.en_ZA
dc.description.urihttp://gbe.oxfordjournals.orgen_ZA
dc.identifier.citationBellieny-Rabelo, D., Pretorius, W.J.S. & Moleleki, L.N. 2021, 'Novel two-component system-like elements reveal functional domains associated with restriction-modification systems and paraMORC ATPases in bacteria', Genome Biology and Evolution, vol. 13, no. 3, pp. 1-15.en_ZA
dc.identifier.issn1759-6653
dc.identifier.other10.1093/gbe/evab024
dc.identifier.urihttp://hdl.handle.net/2263/84391
dc.language.isoenen_ZA
dc.publisherOxford University Pressen_ZA
dc.rights© The Author(s) 2021. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.en_ZA
dc.subjectMORCen_ZA
dc.subjectGHKL ATPasesen_ZA
dc.subjectDNA modification methylasesen_ZA
dc.subjectDNA restriction–modification enzymesen_ZA
dc.subjectHistidine kinaseen_ZA
dc.subjectTwo-component system (TCS)en_ZA
dc.subjectDeoxyribonucleic acid (DNA)en_ZA
dc.titleNovel two-component system-like elements reveal functional domains associated with restriction-modification systems and paraMORC ATPases in bacteriaen_ZA
dc.typeArticleen_ZA

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