Novel two-component system-like elements reveal functional domains associated with restriction-modification systems and paraMORC ATPases in bacteria
dc.contributor.author | Bellieny-Rabelo, Daniel | |
dc.contributor.author | Pretorius, Willem J.S. | |
dc.contributor.author | Moleleki, Lucy Novungayo | |
dc.contributor.email | lucy.moleleki@up.ac.za | en_ZA |
dc.date.accessioned | 2022-03-09T05:39:59Z | |
dc.date.available | 2022-03-09T05:39:59Z | |
dc.date.issued | 2021-03 | |
dc.description.abstract | Two-component systems (TCS) are important types of machinery allowing for efficient signal recognition and transmission in bacterial cells. The majority of TCSs utilized by bacteria is composed of a sensor histidine kinase (HK) and a cognate response regulator (RR). In the present study,we report two newly predicted protein domains—both to be included in the next release of the Pfamdatabase: Response_reg_2 (PF19192) andHEF_HK(PF19191)—in bacteriawhich exhibit high structural similarity, respectively, with typical domains of RRs and HKs. Additionally, the genes encoding for the novel predicted domains exhibit a 91.6% linkage observed across 644 genomic regions recovered from 628 different bacterial strains. The remarkable adjacent colocalization between genes carrying Response_reg_2 and HEF_HK in addition to their conserved structural features, which are highly similar to those fromwell-known HKs and RRs, raises the possibility of Response_reg_2 and HEF_HK constituting a new TCS in bacteria. The genomic regions inwhich these predicted two-component systems-like are located additionally exhibit an overrepresented presence of restriction–modification (R–M) systems especially the type II R–M. Among these, there is a conspicuous presence of C-5 cytosinespecific DNAmethylaseswhichmay indicate a functional associationwith the newly discovered domains. The solid presence of R–M systems and the presence of theGHKL family domain HATPase_c_3 across most of the HEF_HK-containing genes are also indicative that these genes are evolutionarily related to the paraMORC family of ATPases. | en_ZA |
dc.description.department | Biochemistry | en_ZA |
dc.description.department | Forestry and Agricultural Biotechnology Institute (FABI) | en_ZA |
dc.description.department | Genetics | en_ZA |
dc.description.department | Microbiology and Plant Pathology | en_ZA |
dc.description.librarian | am2022 | en_ZA |
dc.description.sponsorship | The National Research Foundation (NRF), the Research Technology Fund and the University of Pretoria Postdoctoral Fellowship. | en_ZA |
dc.description.uri | http://gbe.oxfordjournals.org | en_ZA |
dc.identifier.citation | Bellieny-Rabelo, D., Pretorius, W.J.S. & Moleleki, L.N. 2021, 'Novel two-component system-like elements reveal functional domains associated with restriction-modification systems and paraMORC ATPases in bacteria', Genome Biology and Evolution, vol. 13, no. 3, pp. 1-15. | en_ZA |
dc.identifier.issn | 1759-6653 | |
dc.identifier.other | 10.1093/gbe/evab024 | |
dc.identifier.uri | http://hdl.handle.net/2263/84391 | |
dc.language.iso | en | en_ZA |
dc.publisher | Oxford University Press | en_ZA |
dc.rights | © The Author(s) 2021. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. | en_ZA |
dc.subject | MORC | en_ZA |
dc.subject | GHKL ATPases | en_ZA |
dc.subject | DNA modification methylases | en_ZA |
dc.subject | DNA restriction–modification enzymes | en_ZA |
dc.subject | Histidine kinase | en_ZA |
dc.subject | Two-component system (TCS) | en_ZA |
dc.subject | Deoxyribonucleic acid (DNA) | en_ZA |
dc.title | Novel two-component system-like elements reveal functional domains associated with restriction-modification systems and paraMORC ATPases in bacteria | en_ZA |
dc.type | Article | en_ZA |
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