Mycobacterium malmesburyense sp. nov : a novel non-tuberculous Mycobacterium species revealed by multiple gene sequence characterization

dc.contributor.authorGcebe, Nomakorinte
dc.contributor.authorRutten, Victor P.M.G.
dc.contributor.authorGey van Pittius, Nicolaas
dc.contributor.authorNaicker, Brendon
dc.contributor.authorMichel, Anita Luise
dc.date.accessioned2017-01-30T06:06:38Z
dc.date.available2017-01-30T06:06:38Z
dc.date.issued2017-04
dc.description.abstractNon-tuberculous mycobacteria (NTM) are ubiquitous in the environment and an increasing number of NTM species have been isolated and characterized from both humans and animals, highlighting the zoonotic potential of these bacteria. Host exposure to NTM may impact on cross-reactive immune responsiveness which may affect diagnosis of bovine tuberculosis and may also play a role in the variability of the efficacy of Mycobacterium bovis BCG vaccination against tuberculosis. In this study we characterized 10 NTM isolates originating from water, soil, nasal swabs of cattle and African buffalo as well as bovine tissue samples. These isolates were previously identified during an NTM survey and were all found, using 16S rDNA sequence analysis to be closely-related to Mycobacterium moriokaense. A polyphasic approach that includes phenotypic characterization, antibiotic susceptibility profiling, mycolic acid profiling and phylogenetic analysis of four gene loci, viz 16S rDNA, hsp65, sodA, and rpoB was employed to characterize these isolates. Sequence data analysis of the four gene loci revealed that these isolates belong to a unique Mycobacterium species. This evidence was further supported by several differences in phenotypic characteristics between the isolates and the closely related species. We proposed the name, Mycobacterium malmesburyense sp. nov. for this new species. The type strain is WCM 7299T (ATCC® BAA- 2759TM =CIP 110822T). The Genbank accession numbers for the partial gene sequences [16S rDNA , hsp65, rpoB and sodA] for the type strain are as follows: 16S rRNA= KJ 873241; hsp65=KJ 873243; rpoB= KJ 873245; sodA= KJ 873247.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianhb2017en_ZA
dc.description.sponsorshipWOTRO Science for global development; grant number W01.65.321.00.en_ZA
dc.description.urihttp://ijs.sgmjournals.orgen_ZA
dc.identifier.citationGcebe, N, Rutten, VPMG, Gey van Pittius, N, Naicker, B & Michel, AL 2017, 'Mycobacterium malmesburyense sp. nov: A novel non-tuberculous Mycobacterium species revealed by multiple gene sequence characterization', International Journal of Systematic and Evolutionary Microbiology, vol. 67, no. 4, pp. 832-838.en_ZA
dc.identifier.issn1466-5026 (print)
dc.identifier.issn1466-5034 (online)
dc.identifier.other10.1099/ijsem.0.001678
dc.identifier.urihttp://hdl.handle.net/2263/58673
dc.language.isoenen_ZA
dc.publisherSociety for General Microbiologyen_ZA
dc.rights© 2017 Society for General Microbiology. This is an author manuscript that has been accepted for publication in Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Journal of General Virology, vol. 67, no. 4, pp. 832-838, 2017. doi : 10.1099/ijsem.0.001678.en_ZA
dc.subjectMycobacterium malmesburyense sp. nov.en_ZA
dc.subjectNon-tuberculous mycobacteria (NTM)en_ZA
dc.subjectMultiple gene sequence characterizationen_ZA
dc.titleMycobacterium malmesburyense sp. nov : a novel non-tuberculous Mycobacterium species revealed by multiple gene sequence characterizationen_ZA
dc.typePostprint Articleen_ZA

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