Digestibility and rumen fermentation of a high forage diet pre-treated with a mixture of cellulase and xylanase enzymes

dc.contributor.authorSelzer, Karla
dc.contributor.authorHassen, Abubeker
dc.contributor.authorAkanmu, Abiodun Mayowa
dc.contributor.authorSalem, A.Z.M.
dc.contributor.emailabubeker.hassen@up.ac.zaen_US
dc.date.accessioned2022-10-14T05:24:40Z
dc.date.available2022-10-14T05:24:40Z
dc.date.issued2021-06-19
dc.description.abstractForages play an important role in ruminant animal production worldwide. Unlocking the nutritional potential of poor-quality tropical forages with fibrolytic enzymes would improve forage digestibility and utilization. Using in vitro and in vivo methods this study investigated the effect of pre-treating Smutsfinger hay for 24 hours with a mixture of fibrolytic enzyme (100% cellulase; 75% cellulase: 25% xylanase; 50% cellulase: 50% xylanase; 25% cellulase: 75% xylanase; 100% xylanase and a control with no enzyme) on ruminal fermentation and digestibility of nutrients by sheep. For in vitro fermentation, dry matter, neutral detergent fibre (NDF) degradability and volatile fatty acids (VFA) were determined with standard procedures. The same treatments were used for an in vivo digestibility trial using Merino sheep in a 6 x 6 Latin square design. Feed intake and total tract digestibility were recorded. Rumen fluid samples were collected daily, preserved, and analysed for VFA. The addition of 100% cellulase enzyme to Smutsfinger hay in vitro increased (P <0.05) NDF degradability and gas production compared with the control and inclusion of 100% xylanase enzyme. Both 100% cellulase and xylanase enzymes significantly reduced in vitro end time fermentation pH. A 50:50 mixture of cellulase and xylanase plus enzyme in vivo, increased acetate, total VFA concentration, and higher NDF and ADF digestibility of the test feed compared with the control. Inclusion of a 50-75% mixture of cellulase and 50-25% xylanase enzymes treatment led to higher gas production and butyrate concentration, decreased ruminal pH and improved nutrient digestibilityen_US
dc.description.departmentAnimal and Wildlife Sciencesen_US
dc.description.sponsorshipNational Research Foundationen_US
dc.description.urihttps://www.sasas.co.za/resources/sa-journal-animal-scienceen_US
dc.identifier.citationSelzer, Karla, Hassen, Abubeker, Akanmu, Abiodun & A.Z.M., Salem,. (2021). Digestibility and rumen fermentation of a high forage diet pre-treated with a mixture of cellulase and xylanase enzymes. South African Journal of Animal Science, 51. 1-8. 10.4314/sajas.v51i3.14.en_US
dc.identifier.issn0375-1589 (print)
dc.identifier.issn2221-4062 (online)
dc.identifier.other10.4314/sajas.v51i3.14
dc.identifier.urihttps://repository.up.ac.za/handle/2263/87686
dc.language.isoenen_US
dc.publisherSouth African Society for Animal Scienceen_US
dc.rightsCopyright resides with the authors in terms of the Creative Commons Attribution 4.0 South African License.en_US
dc.subjectFibrolytic enzymesen_US
dc.subjectRuminantsen_US
dc.subjectSheepen_US
dc.subjectVolatile fatty acidsen_US
dc.titleDigestibility and rumen fermentation of a high forage diet pre-treated with a mixture of cellulase and xylanase enzymesen_US
dc.typeArticleen_US

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