An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm)
| dc.contributor.author | Titoy, G.A.P. | |
| dc.contributor.editor | Verwoerd, Daniel Wynand | |
| dc.date.accessioned | 2013-09-12T08:51:12Z | |
| dc.date.available | 2013-09-12T08:51:12Z | |
| dc.date.created | 2013 | |
| dc.date.issued | 1995 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
| dc.description.abstract | A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37°C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0°C in 82,8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69,1% as assessed by motility. | en |
| dc.description.librarian | mn2013 | |
| dc.identifier.citation | Titoy, GAP 1995, 'An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm)’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 3, pp. 215. | en |
| dc.identifier.issn | 0330-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/31704 | |
| dc.language.iso | en | en |
| dc.publisher | Published by the Agricultural Research Council, Onderstepoort Veterinary Institute | en |
| dc.rights | © ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital). | en |
| dc.subject | Veterinary medicine | en |
| dc.subject | Cryopreservation | en |
| dc.subject | Sheathed third-stage larvae | en |
| dc.subject | Gaigeria pachyscelis | en |
| dc.subject | Sandveld hookworm | en |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.title | An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm) | en |
| dc.type | Article | en |