Assessment of Atopobium vaginae and Gardnerella vaginalis concentrations in a cohort of pregnant South African women

dc.contributor.authorRedelinghuys, Mathys J.
dc.contributor.authorEhlers, Marthie Magdaleen
dc.contributor.authorBezuidenhout, J.E.
dc.contributor.authorBecker, Piet J.
dc.contributor.authorKock, Marleen M.
dc.date.accessioned2017-09-13T05:39:06Z
dc.date.issued2017-09
dc.description.abstractOBJECTIVES : The purpose of this cross-sectional study was to assess Atopobium vaginae and Gardnerella vaginalis concentrations in pregnant women of different age groups, gestational age groups, vaginal flora categories and HIV status, and also to determine which DNA concentrations best discriminated between bacterial vaginosis (BV)-positive and non-BV categories. METHODS : Self-collected vaginal swabs were obtained from 220 pregnant women attending an antenatal clinic in Pretoria, Gauteng, South Africa, from July 2012 to December 2012. BV was detected with the Nugent scoring system, and A. vaginae and G. vaginalis DNA was quantified with a multiplex quantitative real-time PCR assay. RESULTS : Median concentrations of A. vaginae and G. vaginalis were not significantly different among various age groups (A. vaginae p=0.98 and G. vaginalis p=0.18) or different trimesters (A. vaginae p=0.31 and G. vaginalis p=0.19), but differed significantly among the vaginal flora categories (A. vaginae p<0.001 and G. vaginalis p<0.001) and HIV status (A. vaginae p<0.001 and G. vaginalis p=0.004). The presence of A. vaginae (OR=5.8; 95% CI 1.34 to 25.21 and p value=0.02) but not that of G. vaginalis (OR=1.90; 95% CI 0.81 to 4.43 and p value=0.14) was associated with HIV infection. An A. vaginae DNA concentration of ≥107 copies/mL together with a positive G. vaginalis result (≥100 copies/mL) best discriminated between BV-positive (39/220) and non-BV categories (181/220) with a sensitivity of 85% (95% CI 0.70 to 0.94) and a specificity of 82% (95% CI 0.76 to 0.88). CONCLUSION : A. vaginae and G. vaginalis were present in high numbers and concentrations in this pregnant cohort. Threshold concentrations should be established for specific populations to ensure sensitive molecular assays for BV detection.en_ZA
dc.description.departmentMedical Microbiologyen_ZA
dc.description.embargo2018-09-30
dc.description.librarianhj2017en_ZA
dc.description.sponsorshipThe University of Pretoria and the Medical Research Council (South Africa).en_ZA
dc.description.urihttp://sti.bmj.comen_ZA
dc.identifier.citationRedelinghuys, M.J., Ehlers, M.M., Bezuidenhoudt, J.E., Becker, P.J. & Kock, M.M. 2017, 'Assessment of Atopobium vaginae and Gardnerella vaginalis concentrations in a cohort of pregnant South African women', Sexually Transmitted Infections, vol. 93, no. 6, pp. 410-415.en_ZA
dc.identifier.issn1368-4973 (print)
dc.identifier.issn1472-3263 (online)
dc.identifier.other10.1136/sextrans-2016-052883
dc.identifier.urihttp://hdl.handle.net/2263/62247
dc.language.isoenen_ZA
dc.publisherBMJ Publishing Groupen_ZA
dc.rightsCopyright Article author (or their employer) 2017. Produced by BMJ Publishing Group Ltd (& RCPCH) under licence.en_ZA
dc.subjectFloraen_ZA
dc.subjectMicrobiomeen_ZA
dc.subjectGardnerella vaginalisen_ZA
dc.subjectAtopobium vaginaeen_ZA
dc.subjectPregnant womenen_ZA
dc.subjectDiagnosisen_ZA
dc.subjectBacterial vaginosis (BV)en_ZA
dc.subjectPolymerase chain reaction (PCR)en_ZA
dc.titleAssessment of Atopobium vaginae and Gardnerella vaginalis concentrations in a cohort of pregnant South African womenen_ZA
dc.typePostprint Articleen_ZA

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