Influence of 2-methoxyestradiol on MCF-7 cells : an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels
dc.contributor.author | Joubert, Annie M. | |
dc.contributor.author | Marais, Sumari | |
dc.contributor.author | Maritz, Christine | |
dc.contributor.email | annie.joubert@up.ac.za | en |
dc.date.accessioned | 2010-02-22T11:06:11Z | |
dc.date.available | 2010-02-22T11:06:11Z | |
dc.date.issued | 2009-02 | |
dc.description.abstract | Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 μM 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the influence of 2-methoxyestradiol on the expression of these proteins may be time- and cell type dependent and thus not evident during the mitotic block observed. Investigation of the regulation of the B-cell lymphoma 2 family will allow researchers to consider signaling pathways for diseases where apoptosis can potentially be controlled. | en |
dc.identifier.citation | Joubert, A, Marais, S & Maritz, C 2009, 'Influence of 2-methoxyestradiol on MCF-7 cells : an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels', Biocell, vol. 33, no. 1, pp. 67-70. [http://www.scielo.org.ar/scielo.php/script_sci_serial/lng_en/pid_0327-9545/nrm_iso ] | en |
dc.identifier.issn | 0327-9545 | |
dc.identifier.uri | http://hdl.handle.net/2263/13203 | |
dc.language.iso | en | en |
dc.publisher | Centro Regional de Investigaciones Cientificas Y Tecnologicas / Universidad Nacional de Cuyo | en |
dc.rights | Centro Regional de Investigaciones Cientificas Y Tecnologicas / Universidad Nacional de Cuyo | en |
dc.subject | Breast carcinoma | en |
dc.subject.lcsh | Breast -- Cancer | en |
dc.subject.lcsh | Mitosis -- Regulation | en |
dc.subject.lcsh | Apoptosis | en |
dc.subject.lcsh | Cell death | en |
dc.subject.lcsh | B cells -- Tumors | en |
dc.subject.lcsh | Lymphomas | en |
dc.subject.lcsh | Proteins | en |
dc.subject.lcsh | 2-methoxyestradiol | en |
dc.title | Influence of 2-methoxyestradiol on MCF-7 cells : an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels | en |
dc.type | Article | en |