Influence of 2-methoxyestradiol on MCF-7 cells : an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels

dc.contributor.authorJoubert, Annie M.
dc.contributor.authorMarais, Sumari
dc.contributor.authorMaritz, Christine
dc.contributor.emailannie.joubert@up.ac.zaen
dc.date.accessioned2010-02-22T11:06:11Z
dc.date.available2010-02-22T11:06:11Z
dc.date.issued2009-02
dc.description.abstractProteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 μM 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the influence of 2-methoxyestradiol on the expression of these proteins may be time- and cell type dependent and thus not evident during the mitotic block observed. Investigation of the regulation of the B-cell lymphoma 2 family will allow researchers to consider signaling pathways for diseases where apoptosis can potentially be controlled.en
dc.identifier.citationJoubert, A, Marais, S & Maritz, C 2009, 'Influence of 2-methoxyestradiol on MCF-7 cells : an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels', Biocell, vol. 33, no. 1, pp. 67-70. [http://www.scielo.org.ar/scielo.php/script_sci_serial/lng_en/pid_0327-9545/nrm_iso ]en
dc.identifier.issn0327-9545
dc.identifier.urihttp://hdl.handle.net/2263/13203
dc.language.isoenen
dc.publisherCentro Regional de Investigaciones Cientificas Y Tecnologicas / Universidad Nacional de Cuyoen
dc.rightsCentro Regional de Investigaciones Cientificas Y Tecnologicas / Universidad Nacional de Cuyoen
dc.subjectBreast carcinomaen
dc.subject.lcshBreast -- Canceren
dc.subject.lcshMitosis -- Regulationen
dc.subject.lcshApoptosisen
dc.subject.lcshCell deathen
dc.subject.lcshB cells -- Tumorsen
dc.subject.lcshLymphomasen
dc.subject.lcshProteinsen
dc.subject.lcsh2-methoxyestradiolen
dc.titleInfluence of 2-methoxyestradiol on MCF-7 cells : an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levelsen
dc.typeArticleen

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Joubert_Influence(2009).pdf
Size:
138.5 KB
Format:
Adobe Portable Document Format
Description:
Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
2.43 KB
Format:
Item-specific license agreed upon to submission
Description: