In vitro study of butyric acid deodorization potential by indigenously constructed bacterial consortia and pure cultures from pit latrine fecal sludge

dc.contributor.authorNjalam'mano, John Bright Joseph
dc.contributor.authorChirwa, Evans M.N.
dc.contributor.authorSeabi, Refilwe Lesego
dc.contributor.emailevans.chirwa@up.ac.zaen_ZA
dc.date.accessioned2021-03-02T08:35:06Z
dc.date.available2021-03-02T08:35:06Z
dc.date.issued2020-06-24
dc.description.abstractThe present study aims at developing an e cient bacterial consortium to biodegrade butyric acid, one of the odor-causing compounds that contribute significantly to pit latrine malodors. Six bacterial strains isolated from pit latrine fecal sludge were selected for the study. Nineteen bacterial consortia of di erent combinations were artificially constructed. The individual bacterial strains and bacterial consortia were compared by culturing in mineral salt medium supplemented with 1000 mg/L butyric acid as a sole carbon and energy source at pH 7, 30 C, and 110 rpm under aerobic growth conditions. A co-culture of Serratia marcescens and Bacillus cereus was an e ective bacterial consortium compared to individual component bacterial strains and other bacterial consortia, in which 1000 mg/L butyric acid was completely degraded within 16 h of incubation. A temperature of 30 C and pH 7 were found to be optimum for the maximum degradation for both S. marcescens and B. cereus. The inoculation sizes of 2.0 and 2.5 were optimal for the maximum degradation for B. cereus and S. marcescens, respectively. The study provides insights that will be of substantial help in the development of e ective biological treatment technologies for pit latrine odor to change the pit latrine user community’s and would be users’ perception of pit latrines.en_ZA
dc.description.departmentChemical Engineeringen_ZA
dc.description.librarianam2021en_ZA
dc.description.sponsorshipThe National Research Foundation (South Africa (NRF) Competitive Programme for Rated Researchers and a postgraduate scholarship through the UP-Commonwealth programme via the Department of Research and Innovation at the University of Pretoria.en_ZA
dc.description.urihttp://www.mdpi.com/journal/sustainabilityen_ZA
dc.identifier.citationNjalam’mano, J.B.T., Chirwa, E.M.N. & Seabi, R.L. 2020, 'In vitro study of butyric acid deodorization potential by indigenously constructed bacterial consortia and pure cultures from pit latrine fecal sludge', Sustainability, vol. 12, art. 5156, pp. 1-18.en_ZA
dc.identifier.issn2071-1050 (online)
dc.identifier.other10.3390/su12125156
dc.identifier.urihttp://hdl.handle.net/2263/78911
dc.language.isoenen_ZA
dc.publisherMDPI Publishingen_ZA
dc.rights© 2020 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_ZA
dc.subjectBacillus cereusen_ZA
dc.subjectBacterial consortiaen_ZA
dc.subjectBiodegradationen_ZA
dc.subjectOdor-causing compoundsen_ZA
dc.subjectSanitationen_ZA
dc.subjectSerratia marcescensen_ZA
dc.titleIn vitro study of butyric acid deodorization potential by indigenously constructed bacterial consortia and pure cultures from pit latrine fecal sludgeen_ZA
dc.typeArticleen_ZA

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