Changes in red blood cell membrane structure in type 2 diabetes : a scanning electron and atomic force microscopy study

dc.contributor.authorBuys, A.V. (Antonia Vergina)
dc.contributor.authorVan Rooy, Mia-Jeanne
dc.contributor.authorSoma, Prashilla
dc.contributor.authorVan Papendorp, D.H. (Dirk Hermanus), 1949-
dc.contributor.authorLipinski, Boguslaw
dc.contributor.authorPretorius, Etheresia
dc.contributor.emailresia.pretorius@up.ac.zaen_ZA
dc.date.accessioned2016-10-31T11:20:23Z
dc.date.available2016-10-31T11:20:23Z
dc.date.issued2013-01-28
dc.description.abstractRed blood cells (RBCs) are highly deformable and possess a robust membrane that can withstand shear force. Previous research showed that in diabetic patients, there is a changed RBC ultrastructure, where these cells are elongated and twist around spontaneously formed fibrin fibers. These changes may impact erythrocyte function. Ultrastructural analysis of RBCs in inflammatory and degenerative diseases can no longer be ignored and should form a fundamental research tool in clinical studies. Consequently, we investigated the membrane roughness and ultrastructural changes in type 2 diabetes. Atomic force microscopy (AFM) was used to study membrane roughness and we correlate this with scanning electron microscopy (SEM) to compare results of both the techniques with the RBCs of healthy individuals. We show that the combined AFM and SEM analyses of RBCs give valuable information about the disease status of patients with diabetes. Effectiveness of treatment regimes on the integrity, cell shape and roughness of RBCs may be tracked, as this cell’s health status is crucial to the overall wellness of the diabetic patient.en_ZA
dc.description.departmentPhysiologyen_ZA
dc.description.librarianam2016en_ZA
dc.description.urihttp://cardiab.biomedcentral.comen_ZA
dc.identifier.citationBuys, AV, Van Rooy, MJ, Soma, P, Van Papendorp, D, Lipinski, B & Pretorius, E 2013, 'Changes in red blood cell membrane structure in type 2 diabetes: a scanning electron and atomic force microscopy study', Cardiovascular Diabetology, vol. 12, art. # 25, pp. 1-6.en_ZA
dc.identifier.issn1475-2840
dc.identifier.other10.1186/1475-2840-12-25
dc.identifier.urihttp://hdl.handle.net/2263/57559
dc.language.isoenen_ZA
dc.publisherBioMed Centralen_ZA
dc.rights© 2013 Buys et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.en_ZA
dc.subjectAtomic force microscopyen_ZA
dc.subjectRed blood cells (RBCs)en_ZA
dc.subjectScanning electron microscopy (SEM)en_ZA
dc.subjectType 2 diabetes mellitus (T2DM)en_ZA
dc.titleChanges in red blood cell membrane structure in type 2 diabetes : a scanning electron and atomic force microscopy studyen_ZA
dc.typeArticleen_ZA

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