Sustained effects of muscle calpain system genotypes on tenderness phenotypes of South African beef bulls during ageing up to 20 days

dc.contributor.authorBasson, Annie
dc.contributor.authorStrydom, Phillip E.
dc.contributor.authorVan Marle-Koster, Este
dc.contributor.authorWebb, E.C. (Edward Cottington)
dc.contributor.authorFrylinck, Lorinda
dc.contributor.emaileste.vanmarle-koster@up.ac.zaen_US
dc.date.accessioned2023-04-05T13:22:50Z
dc.date.available2023-04-05T13:22:50Z
dc.date.issued2022-03-09
dc.description.abstractThe most important factor that determines beef tenderness is its proteolytic activity, and the balance between calpain-1 protease activity and calpastatin inhibition is especially important, while contributions can also arise from calpain-2 and, possibly, calpain-3. The meat ageing process itself affects these processes. To determine whether genotypes in the calpain–calpastatin system can enhance tenderness through a 20-day ageing period, South African purebred beef bulls (n = 166) were genotyped using the Illumina BovineHD SNP BeadChip through a gene-based association analysis targeting the cast, capn3, capn2 and capn1 genes. The Warner–Bratzler shear force (WBSF) and myofibril fragment length (MFL) of Longissimus thoracis et lumborum (LTL) steaks were evaluated between d 3 and d 20 of ageing, with protease enzyme activity in the first 20 h post-mortem. Although several of the 134 SNPs are associated with tenderness, only seven SNP in the cast, capn2 and capn1 genes sustained genetic associations, additive to the ageing-associated increases in tenderness for at least three of the four ageing periods. While most genomic associations were relatively stable over time, some genotypes within the SNP responded differently to ageing, resulting in altered genomic effects over time. The level of ageing at which genomic associations are performed is an important factor that determines whether SNPs affect tenderness phenotypes.en_US
dc.description.departmentAnimal and Wildlife Sciencesen_US
dc.description.librarianam2023en_US
dc.description.sponsorshipThe Red Meat Research and Development South Africa (RMRDSA) (genomic markers in beef tenderness) and the Technology and Human Resources for Industry Programme of the Department of Trade and Industry (DTI THRIP), South Africa.en_US
dc.description.urihttps://www.mdpi.com/journal/animalsen_US
dc.identifier.citationBasson, A.; Strydom, P.E.; van Marle-Köster, E.;Webb, E.C.; Frylinck, L. Sustained Effects of Muscle Calpain System Genotypes on Tenderness Phenotypes of South African Beef Bulls during Ageing up to 20 Days. Animals 2022, 12, 686. https://doi.org/10.3390/ani12060686.en_US
dc.identifier.issn2076-2615 (online)
dc.identifier.other10.3390/ani12060686
dc.identifier.urihttp://hdl.handle.net/2263/90385
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectSNPen_US
dc.subjectCalpain–calpastatin system genesen_US
dc.subjectGenomic associationen_US
dc.subjectTenderizationen_US
dc.subjectAgeingen_US
dc.subjectWarner–Bratzler shear force (WBSF)en_US
dc.subjectLongissimus thoracis et lumborum (LTL)en_US
dc.subjectMyofibril fragment length (MFL)en_US
dc.titleSustained effects of muscle calpain system genotypes on tenderness phenotypes of South African beef bulls during ageing up to 20 daysen_US
dc.typeArticleen_US

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