A comprehensive review on Eryptosis

dc.contributor.authorPretorius, Etheresia
dc.contributor.authorDu Plooy, Jeanette Noel
dc.contributor.authorBester, J.
dc.contributor.emailresia.pretorius@up.ac.zaen_ZA
dc.date.accessioned2017-01-23T07:54:35Z
dc.date.available2017-01-23T07:54:35Z
dc.date.issued2016
dc.description.abstractErythrocytes (RBCs) are extremely sensitive cells, and although they do not have nuclei and mitochondria, are important health indicators. This is particularly true because, during inflammation, whether it is systemic or chronic, the haematological system is constantly exposed to circulating inflammatory mediators. RBCs have a highly specialized and organized membrane structure, which interacts and reacts to inflammatory molecule insults, and undergo programmed cell death, similar to apoptosis, known as eryptosis. Over the past years, eryptosis studies have focussed on determining if membrane changes have occurred, particularly whether a phosphatidylserine (PS) flip, Ca2+ leakage into the cell, changes to ceramide and cell shrinkage have occurred. Mostly, flow cytometry is used, but confocal microscopy and ultrastructural studies also confirm eryptosis. Here, we provide a comprehensive overview of eryptosis, where we revisit the biochemical process of the process, review all literature in PUBMED, that is shown under the search word, “eryptosis”, and also discuss current methodologies to determine the presence of eryptosis; included in the discussion of the methodologies, we discuss a pitfalls section for each method. This paper is therefore a comprehensive synopsis of current knowledge of eryptosis and discusses how RBCs may provide an essential in vivo cell model system to study not only inflammation in disease, but also track disease progression and treatment regimes.en_ZA
dc.description.departmentPhysiologyen_ZA
dc.description.librarianam2017en_ZA
dc.description.sponsorshipNational Research Foundation (NRF) and Medical Research Council (MRC) of South Africa: E Pretorius.en_ZA
dc.description.urihttp://www.karger.com/cpben_ZA
dc.identifier.citationPretorius, E, Du Plooy, JN & Bester, J 2016, 'A comprehensive review on Eryptosis', Cellular Physiology and Biochemistry, vol. 39, no. 5, pp. 1977-2000.en_ZA
dc.identifier.issn1015-8987 (print)
dc.identifier.issn1421-9778 Online)
dc.identifier.other10.1159/00044789
dc.identifier.urihttp://hdl.handle.net/2263/58594
dc.language.isoenen_ZA
dc.publisherKarger Publishers Open Accessen_ZA
dc.rights© 2016 The Author(s) This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).en_ZA
dc.subjectEryptosisen_ZA
dc.subjectMembrane changesen_ZA
dc.subjectFlow cytometryen_ZA
dc.subjectConfocal microscopyen_ZA
dc.subjectScanning electron microscopyen_ZA
dc.subjectErythrocytes (RBCs)en_ZA
dc.titleA comprehensive review on Eryptosisen_ZA
dc.typeBooken_ZA

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