Apoprotein heterogeneity increases spectral disorder and a step-wise modification of the B850 fluorescence peak position

dc.contributor.authorIlioaia, Cristian
dc.contributor.authorKruger, T.P.J. (Tjaart)
dc.contributor.authorIlioaia, Oana
dc.contributor.authorRobert, Bruno
dc.contributor.authorVan Grondelle, Rienk
dc.contributor.authorGall, Andrew
dc.date.accessioned2018-11-02T04:52:23Z
dc.date.issued2018-02
dc.description.abstractIt has already been established that the quaternary structure of the main light-harvesting complex (LH2) from the photosynthetic bacterium Rhodopseudomonas palustris is a nonameric ‘ring’ of PucAB heterodimers and under low-light culturing conditions an increased diversity of PucB synthesis occurs. In this work, single molecule fluorescence emission studies show that different classes of LH2 ‘rings’ are present in “low-light” adapted cells and that an unknown chaperon process creates multiple sub-types of ‘rings’ with more conformational sub-states and configurations. This increase in spectral disorder significantly augments the cross-section for photon absorption and subsequent energy flow to the reaction centre trap when photon availability is a limiting factor. This work highlights yet another variant used by phototrophs to gather energy for cellular development.en_ZA
dc.description.departmentPhysicsen_ZA
dc.description.embargo2019-02-01
dc.description.librarianhj2018en_ZA
dc.description.sponsorshipThe French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INSB-05 (A.G, C.I., B.R); the European Research Council (ERC) through an Advanced Investigator Grant, contract no. 267333 (R.v.G., B.R.); the French Agence Nationale de la Recherch (ANR) through a Chaire d'excellence, ANR-07-CEX-009-01 (A.G., O.I.); the European Union (EU) FP6 MEST-CT-2004-008048 Marie Curie Early Stage Training Network via the Advanced Training in Laser Sciences project (T.P.J.K.); the EU Access to Research Infrastructures action of the Improving Human Potential Program, contract no. RII-CT-2003-506350 (A.G.); CEA-Eurotalents program, European Union contract no. PCOFUND-GA-2008-228664 (C.I.); TOP grant (700.58.305) from the Foundation of Chemical Sciences, part of the Netherlands Organization for Scientific Research (C.I. and R.v.G.).en_ZA
dc.description.urihttp://www.elsevier.com/locate/bbabioen_ZA
dc.identifier.citationIlioaia, C., Kruger, T.P.J., Ilioaia, O. et al. 2018, 'Apoprotein heterogeneity increases spectral disorder and a step-wise modification of the B850 fluorescence peak position', Biochimica et Biophysica Acta (BBA) - Bioenergetics, vol. 1859, no. 2, pp. 137-144.en_ZA
dc.identifier.issn0005-2728
dc.identifier.other10.1016/j.bbabio.2017.11.003
dc.identifier.urihttp://hdl.handle.net/2263/67127
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2017 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta - Bioenergetics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Biochimica et Biophysica Acta - Bioenergetics, vol. 1859, no. 2, pp. 137-144, 2018. doi : 10.1016/j.bbabio.2017.11.003.en_ZA
dc.subjectLight-harvesting complex (LH2)en_ZA
dc.subjectPurple bacteriaen_ZA
dc.subjectSingle molecule spectroscopyen_ZA
dc.subjectPhotosynthesisen_ZA
dc.subjectLow temperatureen_ZA
dc.subjectBacteriochlorophyll (Bchl)en_ZA
dc.subjectRhodopseudomonas palustrisen_ZA
dc.subjectRhodobacter sphaeroidesen_ZA
dc.subjectPigment organizationen_ZA
dc.titleApoprotein heterogeneity increases spectral disorder and a step-wise modification of the B850 fluorescence peak positionen_ZA
dc.typePostprint Articleen_ZA

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