A comparative study of selected physical and biochemical traits of wild-type and transgenic sorghum to reveal differences relevant to grain quality

dc.contributor.authorNdimba, Roya J.
dc.contributor.authorMehlo, Luke
dc.contributor.authorBarnabas, Alban
dc.contributor.authorKissmann, Jens
dc.contributor.authorNdimba, Bongani K.
dc.contributor.upauthorKruger, Johanita
dc.date.accessioned2017-07-25T09:36:13Z
dc.date.available2017-07-25T09:36:13Z
dc.date.issued2017-06-07
dc.description.abstractTransgenic sorghum featuring RNAi suppression of certain kafirins was developed recently, to address the problem of poor protein digestibility in the grain. However, it was not firmly established if other important quality parameters were adversely affected by this genetic intervention. In the present study several quality parameters were investigated by surveying several important physical and biochemical grain traits. Important differences in grain weight, density and endosperm texture were found that serve to differentiate the transgenic grains from their wild-type counterpart. In addition, ultrastructural analysis of the protein bodies revealed a changed morphology that is indicative of the effect of suppressed kafirins. Importantly, lysine was found to be significantly increased in one of the transgenic lines in comparison to wild-type; while no significant changes in anti-nutritional factors could be detected. The results have been insightful for demonstrating some of the corollary changes in transgenic sorghum grain, that emerge from imposed kafirin suppression.en_ZA
dc.description.departmentFood Scienceen_ZA
dc.description.librarianam2017en_ZA
dc.description.urihttp://www.frontiersin.orgen_ZA
dc.identifier.citationNdimba RJ, Kruger J, Mehlo L, Barnabas A, Kossmann J and Ndimba BK (2017) A Comparative Study of Selected Physical and Biochemical Traits of Wild-Type and Transgenic Sorghum to Reveal Differences Relevant to Grain Quality. Front. Plant Sci. 8:952. DOI: 10.3389/fpls.2017.00952.en_ZA
dc.identifier.issn1664-462X
dc.identifier.other10.3389/fpls.2017.00952
dc.identifier.urihttp://hdl.handle.net/2263/61433
dc.language.isoenen_ZA
dc.publisherFrontiers Research Foundationen_ZA
dc.rights© 2017 Ndimba, Kruger, Mehlo, Barnabas, Kossmann and Ndimba. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_ZA
dc.subjectSorghumen_ZA
dc.subjectTransgenicen_ZA
dc.subjectAmino acid profileen_ZA
dc.subjectProtein bodyen_ZA
dc.subjectGrain quality traitsen_ZA
dc.titleA comparative study of selected physical and biochemical traits of wild-type and transgenic sorghum to reveal differences relevant to grain qualityen_ZA
dc.typeArticleen_ZA

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