Characterizing pathology in erythrocytes using morphological and biophysical membrane properties : relation to impaired hemorheology and cardiovascular function in rheumatoid arthritis

dc.contributor.authorOlumuyiwa-Akeredolu, Oore-ofe O.
dc.contributor.authorSoma, Prashilla
dc.contributor.authorBuys, A.V. (Antonia Vergina)
dc.contributor.authorDebusho, Legesse Kassa
dc.contributor.authorPretorius, Etheresia
dc.date.accessioned2018-02-01T08:45:21Z
dc.date.issued2017-12
dc.description.abstractThe inflammatory burden of the complex rheumatoid arthritis (RA) disease affects several organ-systems, including rheological properties of blood and its formed elements. Red blood cells (RBCs) are constantly exposed to circulating dysregulated inflammatory molecules that are co-transported within the vasculature; and their membranes may be particularly vulnerable to the accompanying oxidative stress. In the current study, we investigate biophysical and ultrastructural characteristics of RBCs obtained from a cohort of patients using atomic force microscopy (AFM), scanning electron microscopy (SEM) and confocal microscopy (CM). Statistical analyses of AFM data showed that RA RBCs possessed significantly reduced membrane elasticity relative to that of RBCs from healthy individuals (P-value < 0.0001). SEM imaging of RA RBCs revealed increased anisocytes and poikilocytes. Poikilocytes included knizocytes, stomatocytes, dacryocytes, irregularly contracted cells, and knot cells. CM imaging of several RA RBCs, spectrin, and band 3 protein networks portrayed the similar morphological profiles. Analyses of CM images confirmed changes to distribution of band-3 skeletal protein, a protein critical for gaseous exchange functions of the RBC and preventing membrane surface loss. Decreased membrane deformability impairs the RBC's capacity to adequately adapt its shape to navigate blood vessels, especially microvasculature, and this decrease is also reflected in the cell's morphology. Changes to morphology and deformability may also indicate loss of functional domains and/or pathological protein and lipid associations. These findings suggest that RA disease and/or its concomitant factors impact on the RBC and its membrane integrity with potential for exacerbating pathological cellular function, hemorheology, and cardiovascular function.en_ZA
dc.description.departmentAnatomy and Physiologyen_ZA
dc.description.departmentPhysiologyen_ZA
dc.description.departmentStatisticsen_ZA
dc.description.embargo2018-12-15
dc.description.librarianhj2018en_ZA
dc.description.sponsorshipMedical Research Council: E Pretorius (fund number A0X331) and National Research Foundation: E Pretorius (fund number: N00345).en_ZA
dc.description.urihttp://:www.elsevier.com/locate/bbamemen_ZA
dc.identifier.citationOlumuyiwa-Akeredolu, O.O., Soma, P., Buys, A.V. et al. 2017, 'Characterizing pathology in erythrocytes using morphological and biophysical membrane properties : relation to impaired hemorheology and cardiovascular function in rheumatoid arthritis', Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1859, no. 12, pp. 2381-2391.en_ZA
dc.identifier.issn0005-2736
dc.identifier.other10.1016/j.bbamem.2017.09.014
dc.identifier.urihttp://hdl.handle.net/2263/63840
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2017 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta - Biomembranes. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Biochimica et Biophysica Acta - Biomembranes, vol. 1859, no. 12, pp. 2381-2391, 2017. doi : 10.1016/j.bbamem.2017.09.014.en_ZA
dc.subjectRed blood cells (RBCs)en_ZA
dc.subjectRheumatoid arthritis (RA)en_ZA
dc.subjectAtomic force microscopy (AFM)en_ZA
dc.subjectScanning electron microscopy (SEM)en_ZA
dc.subjectConfocal microscopy (CM)en_ZA
dc.subjectRheologyen_ZA
dc.subjectPoikilocytesen_ZA
dc.subjectMembraneen_ZA
dc.subjectErythrocyteen_ZA
dc.titleCharacterizing pathology in erythrocytes using morphological and biophysical membrane properties : relation to impaired hemorheology and cardiovascular function in rheumatoid arthritisen_ZA
dc.typePostprint Articleen_ZA

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