The development of an ELISA-assay for semi-quantitative detection of dihydrogriesenin, a sesquiterpene lactone from Geigeria

dc.contributor.authorBornman, L.
dc.contributor.authorVermeulen, N.M.J.
dc.contributor.editorBigalke, R.D.
dc.contributor.upauthorVerschoor, Jan Adrianus
dc.date.accessioned2014-11-17T08:32:36Z
dc.date.available2014-11-17T08:32:36Z
dc.date.created2014
dc.date.issued1987
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en_US
dc.description.abstractCertain species of Geigeria contain sesquiterpene lactones which cause vomiting disease in sheep. Dihydrogriesenin (DHG), a sesquiterpene lactone from G. aspera, contains an α-methylene function which can spontaneously react with thiol groups on proteins to form a covalent adduct. A specific antiserum against a DHG-protein adduct can be used to determine the fate of DHG in poisoned animals. The preparation of such an antiserum is reported in this paper. DHG was reacted with cysteine and subsequently coupled to serum albumin using the carbodiimide reaction. When rabbits were immunized with one such conjugate (DHG-bovine serum albumin), it was found that the carrier determinants were immunodominant. A DHG-specific anti-serum of sufficient (ELISA) titre could, however, be obtained by alternating serum albumin carriers for DHG in booster immunizations. The ELISA antigen-antibody reaction could be inhibited by prior reaction of the antisera with cysteinyl-DHG in solution.en_US
dc.description.librarianlmchunu2014en_US
dc.description.librarianmn2014
dc.identifier.citationVerschoor, J.A., Bornman, L. & Vermeulen, N.M.J. 1987, 'The development of an ELISA-assay for semi-quantitative detection of dihydrogriesenin, a sesquiterpene lactone from Geigeria’, Onderstepoort Journal of Veterinary Research, vol. 54, no. 1, pp. 1-4.en_US
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/42587
dc.language.isoenen_US
dc.publisherPublished by the Government Printer, Pretoria.en_US
dc.rights©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).en_US
dc.subjectVeterinary medicineen_US
dc.subject.lcshVeterinary medicine -- South Africa
dc.titleThe development of an ELISA-assay for semi-quantitative detection of dihydrogriesenin, a sesquiterpene lactone from Geigeriaen_US
dc.typeArticleen_US

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