Development of polymorphic microsatellite markers for the genetic characterisation of Knoxdaviesia proteae (Ascomycota: Microascales) using ISSR-PCR and pyrosequencing

dc.contributor.authorAylward, Janneke
dc.contributor.authorDreyer, Leanne Laurette
dc.contributor.authorSteenkamp, Emma Theodora
dc.contributor.authorWingfield, Michael J.
dc.contributor.authorRoets, Francois
dc.date.accessioned2014-07-08T08:15:25Z
dc.date.available2014-07-08T08:15:25Z
dc.date.issued2014-05
dc.description.abstractKnoxdaviesia proteae is one of the first native ophiostomatoid fungi discovered in South Africa, where it consistently occurs in the infructescences of the iconic Cape Biome plant, Protea repens. Although numerous studies have been undertaken to better understand the ecology of K. proteae, many questions remain to be answered, particularly given its unique niche and association with arthropods for dispersal.We describe the development and distribution of microsatellite markers in K. proteae through Interspersed Simple Sequence Repeat-Polymerase Chain Reaction (ISSR-PCR) enrichment and pyrosequencing. A large proportion of the 31492 sequences obtained from sequencing the enriched genomic DNA were characterised by microsatellites consisting of short tandem repeats and di- and tri-nucleotide motifs. Seventeen percent of these microsatellites contained flanking regions sufficient for primer design. Twenty-three primer pairs were tested, of which 12 amplified and 10 generated polymorphic fragments in K. proteae. Half of these could be transferred to the sister species, K. capensis. The developedmarkers will be used to investigate the reproductive system, genetic diversity and dispersal strategies of K. proteae.en_US
dc.description.librarianhb2014en_US
dc.description.sponsorshipNational Research Foundation (NRF) and the Department of Science and Technology (DST)/NRF Centre of Excellence in Tree Health Biotechnology (CTHB).en_US
dc.description.urihttp://link.springer.com/journal/11557en_US
dc.identifier.citationAylward, J, Dreyer, LL, Steenkamp, ET, Wingfield, MJ & Roets, F 2014, 'Development of polymorphic microsatellite markers for the genetic characterisation of Knoxdaviesia proteae (Ascomycota: Microascales) using ISSR-PCR and pyrosequencing', Mycological Progress, vol. 13. no. 2, pp. 439-444.en_US
dc.identifier.issn1617-416X (print)
dc.identifier.issn1861-8952 (online)
dc.identifier.other10.1007/s11557-013-0951-1
dc.identifier.urihttp://hdl.handle.net/2263/40623
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rights© German Mycological Society and © Springer-Verlag Berlin Heidelberg 2014. The original publication is available at : http://link.springer.com/journal/11557.en_US
dc.subjectKnoxdaviesiaen_US
dc.subjectMicrosatellitesen_US
dc.subjectOphiostomatoiden_US
dc.subjectPyrosequencingen_US
dc.subjectInterspersed Simple Sequence Repeat-Polymerase Chain Reaction (ISSR-PCR)en_US
dc.titleDevelopment of polymorphic microsatellite markers for the genetic characterisation of Knoxdaviesia proteae (Ascomycota: Microascales) using ISSR-PCR and pyrosequencingen_US
dc.typePostprint Articleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Aylward_Development_2014.pdf
Size:
370.99 KB
Format:
Adobe Portable Document Format
Description:
Postprint Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: