Detection of anaplasma antibodies in wildlife and domestic species in wildlife-livestock interface areas of Kenya by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay

dc.contributor.authorNgeranwa, J.J.N.
dc.contributor.authorShompole, S.P.
dc.contributor.authorVenter, Estelle Hildegard
dc.contributor.authorWambugu, A.
dc.contributor.authorCrafford, Jan Ernst
dc.contributor.authorPenzhorn, Barend Louis
dc.coverage.spatialAfricaen
dc.coverage.spatialKenyaen
dc.date.accessioned2009-04-19T08:03:43Z
dc.date.available2009-04-19T08:03:43Z
dc.date.issued2008-09
dc.description.abstractThe seroprevalence of Anaplasma antibodies in wildlife (eland, blue wildebeest, kongoni, impala, Thomson's gazelle, Grant's gazelle, giraffe and plains zebra) and domestic animal (cattle, sheep and goat) populations was studied in wildlife / livestock interface areas of Kenya. Serum samples were analyzed by competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA), using a recombinant antigen (MSP-5) from Anaplasma marginale surface membrane. A monoclonal antibody, FC-16, was used as the primary antibody, while anti-mouse conjugated to horseradish peroxidase was used as the secondary antibody. The results indicate a high seroprevalence in both wildlife and livestock populations, in contrast to earlier reports from Kenya, which indicated a low seroprevalence. The differences are attributed to the accurate analytical method used (CI-ELISA), as compared with agglutination techniques, clinical signs and microscopy employed by the earlier workers.en
dc.description.sponsorshipNetherlands Government,through the Wildlife Disease Research Project and the Director, Kenya Agricultural Research Institute (KARl)en
dc.identifier.citationNgeranwa, JJN, Shompole, SP, Venter, EH, Wambugu, A, Crafford, JE & Penzhorn, BL 2008, ‘Detection of Anaplasma antibodies in wildlife and domestic species in wildlife-livestock interface areas of Kenya by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay’, Onderstepoort Journal of Veterinary Research, vol. 75, no.3, pp.199–205. [http://www.journals.co.za/ej/ejour_opvet.html]en
dc.identifier.issn0030-2465
dc.identifier.other35609781000
dc.identifier.other7003904650
dc.identifier.otherJ-7222-2013
dc.identifier.otherO-6953-2014
dc.identifier.urihttp://hdl.handle.net/2263/9702
dc.language.isoenen
dc.publisherAgricultural Research Council, ARC-OVI and the University of Pretoriaen
dc.relation.requiresAdobe Acrobat Readeren
dc.rightsAgricultural Research Council, ARC-OVI and the University of Pretoriaen
dc.subjectCI-ELISAen
dc.subjectSeroprevalenceen
dc.subjectWildlife-livestock interfaceen
dc.subjectELISAen
dc.subject.lcshAnaplasmaen
dc.subject.lcshAnimals -- Kenyaen
dc.subject.lcshDomestic animals -- Kenyaen
dc.subject.lcshEnzyme-linked immunosorbent assayen
dc.titleDetection of anaplasma antibodies in wildlife and domestic species in wildlife-livestock interface areas of Kenya by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assayen
dc.typeArticleen

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