Four quantitative trait loci associated with low Nosema ceranae (Microsporidia) spore load in the honeybee Apis mellifera

dc.contributor.authorHuang, Qiang
dc.contributor.authorKryger, Per
dc.contributor.authorConte, Yves
dc.contributor.authorLattorff, H.Michael G.
dc.contributor.authorKraus, F. Bernhard
dc.contributor.authorMoritz, Robin F.A.
dc.date.accessioned2015-11-13T07:24:38Z
dc.date.available2015-11-13T07:24:38Z
dc.date.issued2014-03
dc.description.abstractNosema ceranae has been recently introduced into the honeybee Apis mellifera as a novel microsporidian gut parasite. To locate the genetic region involved in N. ceranae infection tolerance, we fed N. ceranae spores to haploid drones of a F1 hybrid queen produced from a cross between a queen of a Nosemaresistant bred strain and drones of susceptible colonies. The spore loads of the infected F1 drones were used as the phenotype to identify quantitative trait loci (QTLs) associated with N. ceranae spore load. One hundred forty-eight infected drones were individually genotyped with microsatellite markers at an average marker distance of 20 cM along the genome. Four QTLs were significantly associated with low spore load, explaining 20.4 % of total spore load variance. Moreover, a candidate gene Aubergine (Aub) within the major QTL region was significantly overexpressed in drones with low spore loads than in those with high spore loads. Our results confirm the genetic basis of Nosema tolerance in the selected strain and show that both additive effects and epistatic interactions among the QTLs interfere with the tested phenotype.en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipEuropean Union’s Seventh Framework Programme (FP7/2007-2013) under grant agreement BEE DOC FP7-KBBE-2009-3 244956CPFP, the Deutsche Forschungsgemeinschaft Project DFG SPP 1399(MO 373/26-1) and the China Scholarship Council.en_ZA
dc.description.urihttp://link.springer.com/journal/13592en_ZA
dc.identifier.citationHuang, Q, Kryger, P, Le Conte, Y, Lattorff, HMG, Kraus, FB & Moritz, RFA 2014, 'Four quantitative trait loci associated with low Nosema ceranae (Microsporidia) spore load in the honeybee Apis mellifera', Apidologie, vol. 45, no. 2, pp. 248-256.en_ZA
dc.identifier.issn0044-8435 (print)
dc.identifier.issn1297-9678 (online)
dc.identifier.otherhttp://dx.doi.org/10.1007/s13592-013-0243-4
dc.identifier.urihttp://hdl.handle.net/2263/50453
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© INRA, DIB and Springer-Verlag France, 2013. The original publication is available at : http://link.springer.comjournal/13592.en_ZA
dc.subjectApis melliferaen_ZA
dc.subjectDroneen_ZA
dc.subjectNosemaen_ZA
dc.subjectHoneybee (Apis mellifera)en_ZA
dc.subjectQuantitative trait loci (QTL)en_ZA
dc.titleFour quantitative trait loci associated with low Nosema ceranae (Microsporidia) spore load in the honeybee Apis melliferaen_ZA
dc.typePostprint Articleen_ZA

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