Exo-β-1,3-glucanase from yeast inhibits Colletotrichum lupini and Botrytis cinerea spore germination

dc.contributor.authorOelofse, Dean
dc.contributor.authorDubery, Ian A.
dc.contributor.authorBerger, David Kenneth
dc.date.accessioned2009-05-07T05:32:48Z
dc.date.available2009-05-07T05:32:48Z
dc.date.issued2009
dc.description.abstractYeast exo-β-1,3-glucanase (EXG1) was evaluated as an inhibitory agent of Colletotrichum lupini and Botrytis cinerea. Extracts obtained from yeast transformed with the exg1 gene, expressing high levels of EXG1 activity, or control untransformed yeast cultures that lacked EXG1 activity, were added to different starting concentrations of C. lupini fungal spore suspensions (2.5 × 103 to 80 × 103 spores per flask), and mycelial dry weight was measured after 5 days. Inhibition of C. lupini mycelial growth by EXG1 compared with control extracts ranged from 41 to 20% when added to starting fungal spore concentrations of 2.5 × 103 to 80 × 103, respectively. EXG1 activity in the extracts from the transformed yeast remained high over the 5-day incubation period. Addition of the EXG1 extract after C. lupini spore germination resulted in lower inhibition, indicating that the EXG1 targets the β-glucan in the cell walls of the fungal spores at an early stage of germination. Furthermore, the yeast EXG1 extracts were also shown to inhibit Botrytis cinerea spore germination and growth. Thus, the use of the yeast exg1 gene for protection of crops, such as lupin and pear in transgenic strategies against C. lupini and B. cinerea, respectively, could be considered.en_US
dc.identifier.citationOelofse, D, Dubery, I & Berger, DK 2007, ‘Exo-β-1,3-Glucanase from Yeast Inhibits Colletotrichum lupini and Botrytis cinerea Spore Germination’, Journal of Phytopathology, vol.157, no.1, pp. 1-6. [http://www3.interscience.wiley.com/journal/118496757/home]en_US
dc.identifier.issn1439-0434
dc.identifier.other10.1111/j.1439-0434
dc.identifier.urihttp://hdl.handle.net/2263/9918
dc.language.isoenen_US
dc.publisherBlackwellen_US
dc.rightsBlackwell. The definitive version is available at www.blackwell-synergy.com.en_US
dc.subjectColletotrichum lupinien_US
dc.subjectBotrytis cinereaen_US
dc.subjectAnti-fungalen_US
dc.subjectExo-β-1,3-glucanaseen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.titleExo-β-1,3-glucanase from yeast inhibits Colletotrichum lupini and Botrytis cinerea spore germinationen_US
dc.typePostprint Articleen_US

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